Square-Wave Stripping Voltammetric Analysis of Human Papilloma Virus 58 DNA on Carbon Fiber Microelectrode

Abstract

Electrochemical behaviour of polymerase chain reaction (PCR) products of human papilloma virus (HPV) 58 DNA was studied employing cyclic voltammetry (CV) and square-wave stripping voltammetry (SWSV) on a carbon fiber micro-electrode (CFME) in order to develop a simple electroanalytical method to detect and determine HPV 58. This is to seek a possible substitute method for the common technique of gel electrophoresis and fluorescent image analysis for its detection. An anodic stripping peak that appears at -0.6 V (versus Ag/AgCl) was utilized for the determination. Optimum conditions for the square-wave adsorptive stripping analysis were found to be -1.0 V of deposition potential, 90 s of deposition time, 150 mV of SW amplitude, 4 mV of step potential and 120 Hz of SW frequency. A linear curve was obtained in a concentration range of 0.01-0.23 mg/L. The relative standard deviations at 0.01, 0.05 and 0.09 mg/L of concentration were 3.1, 2.7 and 5.3 % (n = 5), respectively at the optimum conditions. The detection limit (S/N) was found to be 0.006 mg/L. The method was applied to determine HPV 58 DNA from a real sample from a patient infected for the diagnostic purpose.