Determination of Fat Soluble Vitamins E, D2, A, K in Syrian Pharmaceutical Preparations by High Performance Liquid Chromatography with UV-DAD Detection

Abstract

HPLC method was developed and applied to the determination of fat soluble vitamins FSV (E, A, K, D₂) in Syrian pharmaceutical preparations in three different columns. High performance liquid chromatography with UV-DAD detection was employed. The developed chromatographic method employed an ODS, CN and C₈ columns with dimension for each (250 mm × 4.6 mm; 5 μm). Solvent systems were employed depending on the expected column type in the studied samples. With first column the mobile phase was (methanol: dichloromethane). For the second column the mobile phase was (n-hexane:isopropanol). finally with third column it was (water:methanol:acetonitrile). This new method has offered a determination of fat soluble vitamins (E, A, K, D₂) directly with a high accuracy and authenticity for the results without extraction need.