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Antioxidant Potential and Xanthine Oxidase Inhibitory Activity of Croton sparsiflorus
Asian Journal of Chemistry,
Vol. 22 No. 1 (2010): Vol 22 Issue 1, 2010
Abstract
Croton sparsiflorus (traditional treatment for cuts, ulcer, fever, inflammation,
hypertension, analgesic and as antivenom) extracts were examined
for xanthine oxidase inhibitory (XOI) potential, antioxidant activity
(DPPH• and phosphomolybdenum assays) and total phenolics. Dichloromethane
(CSD), chloroform (CSC), ethyl acetate (CSEA), acetone
(CSA), ethanol (CSE) and n-butanol (CSB) extracts inhibited xanthine
oxidase > 50 % at 100 μg/mL concentration with IC50 values of 23.82,
17.34, 9.31, 11.35, 43.25 and 87.50 μg/mL, respectively. CSEA, CSM
(methanolic extracts) and CSE extract exhibited significant DPPH• free
radical scavenging potential. Total phenolics; estimated as gallic acid
equivalents (mg/g of the extract); ranged from 28 to 483 (CSC, CSEA
and CSA > 400 mg/g). A weak correlation of total phenolics with XOI
and phosphomolybdenum assays (R2 = 0.5413 and 0.4975, respectively)
proved phenolics are not the only bioactive compounds responsible for
these activities. Strong correlation between total phenolics and DPPH•
free radical scavenging (R2 = 0.8485) marked phenolics as the major
free radical scavengers.
hypertension, analgesic and as antivenom) extracts were examined
for xanthine oxidase inhibitory (XOI) potential, antioxidant activity
(DPPH• and phosphomolybdenum assays) and total phenolics. Dichloromethane
(CSD), chloroform (CSC), ethyl acetate (CSEA), acetone
(CSA), ethanol (CSE) and n-butanol (CSB) extracts inhibited xanthine
oxidase > 50 % at 100 μg/mL concentration with IC50 values of 23.82,
17.34, 9.31, 11.35, 43.25 and 87.50 μg/mL, respectively. CSEA, CSM
(methanolic extracts) and CSE extract exhibited significant DPPH• free
radical scavenging potential. Total phenolics; estimated as gallic acid
equivalents (mg/g of the extract); ranged from 28 to 483 (CSC, CSEA
and CSA > 400 mg/g). A weak correlation of total phenolics with XOI
and phosphomolybdenum assays (R2 = 0.5413 and 0.4975, respectively)
proved phenolics are not the only bioactive compounds responsible for
these activities. Strong correlation between total phenolics and DPPH•
free radical scavenging (R2 = 0.8485) marked phenolics as the major
free radical scavengers.
Keywords
sp DPPH
Euphorbiaceae
Croton bonplandianus
Phosphomolybdenum
Antioxidant activity
Total phenols.
AHMAD, N., SHAHWAR, D., AKMAL KHAN*, M., & SHARIF MUGHAL†, M. (2009). Antioxidant Potential and Xanthine Oxidase Inhibitory Activity of Croton sparsiflorus. Asian Journal of Chemistry, 22(1), 555–561. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/16677
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