Colorimetric Determination of Baclofen with Ninhydrin Reagent and Compare with HPLC Method in Tablet

Abstract

A simple and sensitive spectrophotometeric method has been developed

for the determination of baclofen in tablet. Baclofen reacts with

ninhydrin reagent in aqueous solution producing a coloured product.

In this study, the different experimental parameters affecting the development

and stability of the colour were carefully studied and optimized.

The modified HPLC with ion pair method was used for quantification

of baclofen in the same tablets that used with spectrophotometery. The

absorbance-concentration plot is linear over the range 0.4-1.4 mg/mL

with correlation coefficient of 0.995. The molar absorptive was 283.36

mol/L cm. The validity of the described procedures was assessed to

show that this method had good validity at different times. Statistical

analysis of the results has been carried out with F-test and T-test showed

that it has high accuracy with HPLC method, but it has not good precision

compared to HPLC method.