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Colorimetric Determination of Baclofen with Ninhydrin Reagent and Compare with HPLC Method in Tablet
Asian Journal of Chemistry,
Vol. 22 No. 1 (2010): Vol 22 Issue 1, 2010
Abstract
A simple and sensitive spectrophotometeric method has been developed
for the determination of baclofen in tablet. Baclofen reacts with
ninhydrin reagent in aqueous solution producing a coloured product.
In this study, the different experimental parameters affecting the development
and stability of the colour were carefully studied and optimized.
The modified HPLC with ion pair method was used for quantification
of baclofen in the same tablets that used with spectrophotometery. The
absorbance-concentration plot is linear over the range 0.4-1.4 mg/mL
with correlation coefficient of 0.995. The molar absorptive was 283.36
mol/L cm. The validity of the described procedures was assessed to
show that this method had good validity at different times. Statistical
analysis of the results has been carried out with F-test and T-test showed
that it has high accuracy with HPLC method, but it has not good precision
compared to HPLC method.
for the determination of baclofen in tablet. Baclofen reacts with
ninhydrin reagent in aqueous solution producing a coloured product.
In this study, the different experimental parameters affecting the development
and stability of the colour were carefully studied and optimized.
The modified HPLC with ion pair method was used for quantification
of baclofen in the same tablets that used with spectrophotometery. The
absorbance-concentration plot is linear over the range 0.4-1.4 mg/mL
with correlation coefficient of 0.995. The molar absorptive was 283.36
mol/L cm. The validity of the described procedures was assessed to
show that this method had good validity at different times. Statistical
analysis of the results has been carried out with F-test and T-test showed
that it has high accuracy with HPLC method, but it has not good precision
compared to HPLC method.
Keywords
m Baclofen
Colorimetry
Spectrophotomery
Ninhydrin.
JALAL HOSSEINIMEHR*, S., POURMORAD, F., MOSHTAGHI, E., & AMINI†, M. (2024). Colorimetric Determination of Baclofen with Ninhydrin Reagent and Compare with HPLC Method in Tablet. Asian Journal of Chemistry, 22(1), 522–526. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/16673
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