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Comparison Between The Experimentally and Numerically Modeled and Simulated Kinetic Parameters Corresponding to Michaelis-Menten Equation for NADH Sensors Based on Polymeric Phenothiazine Modified Electrodes
Asian Journal of Chemistry,
Vol. 22 No. 1 (2010): Vol 22 Issue 1, 2010
Abstract
The paper presents a comparison between the experimentally and
numerical modeled and simulated kinetic parameters (Michaelis-Menten
constant, KM and Imax) obtained by fitting the Michaelis-Menten equation,
for NADH amperometric measurements at electrodes modified with
polymeric phenothiazines. The modified electrodes were obtained by
two different approaches: (i) adsorption of the poly-phenothiazine formaldehyde
(PPF) on graphite electrodes (G/PPF) and (ii) electro polymerization
of bis-phenothiazin-3-yl-methane (BPhM) on glassy carbon electrodes
(GC/poly-BPhM). It was obtained a good correlation of the simulated
kinetic parameters with the experimental values. GC/poly-BPhM electrodes
present a higher sensitivity for NADH detection than G/PPF electrodes,
at an applied potential of +200 mV vs. SCE and +50 mV vs. SCE,
respectively.
numerical modeled and simulated kinetic parameters (Michaelis-Menten
constant, KM and Imax) obtained by fitting the Michaelis-Menten equation,
for NADH amperometric measurements at electrodes modified with
polymeric phenothiazines. The modified electrodes were obtained by
two different approaches: (i) adsorption of the poly-phenothiazine formaldehyde
(PPF) on graphite electrodes (G/PPF) and (ii) electro polymerization
of bis-phenothiazin-3-yl-methane (BPhM) on glassy carbon electrodes
(GC/poly-BPhM). It was obtained a good correlation of the simulated
kinetic parameters with the experimental values. GC/poly-BPhM electrodes
present a higher sensitivity for NADH detection than G/PPF electrodes,
at an applied potential of +200 mV vs. SCE and +50 mV vs. SCE,
respectively.
Keywords
thiazine derivatives
NADH
Numerical modeling
Simulation
Michaelis-Menten equation.
LIGIA UNGURESAN*, M., & MARIA GLIGOR†, D. (2009). Comparison Between The Experimentally and Numerically Modeled and Simulated Kinetic Parameters Corresponding to Michaelis-Menten Equation for NADH Sensors Based on Polymeric Phenothiazine Modified Electrodes. Asian Journal of Chemistry, 22(1), 475–482. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/16667
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