Comparison Between The Experimentally and Numerically Modeled and Simulated Kinetic Parameters Corresponding to Michaelis-Menten Equation for NADH Sensors Based on Polymeric Phenothiazine Modified Electrodes

Abstract

The paper presents a comparison between the experimentally and

numerical modeled and simulated kinetic parameters (Michaelis-Menten

constant, K_M and I_max) obtained by fitting the Michaelis-Menten equation,

for NADH amperometric measurements at electrodes modified with

polymeric phenothiazines. The modified electrodes were obtained by

two different approaches: (i) adsorption of the poly-phenothiazine formaldehyde

(PPF) on graphite electrodes (G/PPF) and (ii) electro polymerization

of bis-phenothiazin-3-yl-methane (BPhM) on glassy carbon electrodes

(GC/poly-BPhM). It was obtained a good correlation of the simulated

kinetic parameters with the experimental values. GC/poly-BPhM electrodes

present a higher sensitivity for NADH detection than G/PPF electrodes,

at an applied potential of +200 mV vs. SCE and +50 mV vs. SCE,

respectively.