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Chemical Constituents of Carissa opaca Extracts and Their Evaluation as Antioxidants and Preservatives in Edible Oils
Asian Journal of Chemistry,
Vol. 22 No. 1 (2010): Vol 22 Issue 1, 2010
Abstract
Carissa opaca (Stapf ex Haines) leaves were extracted using Soxhlet
apparatus with petroleum ether (COE-PE), chloroform (COE-CH), ethyl
acetate (COE-EA), acetone (COE-AC), methanol (COE-ME), n-butanol
(COE-BU) and water (COE-WA). Antioxidant potential of Carissa
opaca extracts (COEs) was evaluated by four methods: ferric thiocyanate,
thiobarbituric acid, scavenging of DPPH• and total antioxidant capacity
along with determination of total phenolics and antioxidant potential of
COEs in peanut and sunflower oils. Results revealed that COEs had
significant antioxidant potential (inhibition of lipid peroxidation > 50 %)
and DPPH· scavenging potential (scavenging > 80 %). Moreover, total
phenolic content was also higher in the extracts: COE-AC = 940 and
COE-BU = 600 mg of gallic acid equivalents (GAE). Application of
COEs as natural antioxidants in peanut and sunflower oils significantly
reduced lipid peroxidation (inhibition > 60 %). A strong correlation of
total phenols was observed with DPPH• and total antioxidant capacity
assays (R2 = 0.9776 and R2 = 0.8104, respectively).
apparatus with petroleum ether (COE-PE), chloroform (COE-CH), ethyl
acetate (COE-EA), acetone (COE-AC), methanol (COE-ME), n-butanol
(COE-BU) and water (COE-WA). Antioxidant potential of Carissa
opaca extracts (COEs) was evaluated by four methods: ferric thiocyanate,
thiobarbituric acid, scavenging of DPPH• and total antioxidant capacity
along with determination of total phenolics and antioxidant potential of
COEs in peanut and sunflower oils. Results revealed that COEs had
significant antioxidant potential (inhibition of lipid peroxidation > 50 %)
and DPPH· scavenging potential (scavenging > 80 %). Moreover, total
phenolic content was also higher in the extracts: COE-AC = 940 and
COE-BU = 600 mg of gallic acid equivalents (GAE). Application of
COEs as natural antioxidants in peanut and sunflower oils significantly
reduced lipid peroxidation (inhibition > 60 %). A strong correlation of
total phenols was observed with DPPH• and total antioxidant capacity
assays (R2 = 0.9776 and R2 = 0.8104, respectively).
Keywords
E- Constituents
Carissa opaca
Peanut oil
Sunflower oil
Antioxidant activity.
AKMAL KHAN*, M., SHAHWAR, D., AHMAD, N., KHAN†, Z.-U.-D., & AJAIB†, M. (2009). Chemical Constituents of Carissa opaca Extracts and Their Evaluation as Antioxidants and Preservatives in Edible Oils. Asian Journal of Chemistry, 22(1), 379–388. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/16658
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