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Antioxidant Potential Profile of Extracts from Different Parts of Black Mulberry
Asian Journal of Chemistry,
Vol. 22 No. 1 (2010): Vol 22 Issue 1, 2010
Abstract
Present study is aimed to exploit a potent and cheaper natural sources of
antioxidants. Morus nigra L. is considered to be an antidiabetic and diuretic
agent in the folk medicinal system of Indo-Pak subcontinent. Antioxidant
potential and radical scavenging activity of the various organic and aqueous
extracts of leaves, stem and fruit of M. nigra were investigated using 2,2'-
azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) decolourization
assay, ferric reducing antioxidant power (FRAP) assay, 2.2'-diphenyl-1-picrylhydrazyl
(DPPH) assay, total phenolic content (TPC) assay and total antioxidant
activity assay. Using ABTS•+ decolourization assay and FRAP assay, M.
nigra extracts showed a wide range of antioxidant activity. Using TPC assay
the amount of total phenolics ranged from 1.3048 to 5.8287, 0.413 to 2.9394
and 0.5346 to 6.2266 mg/L for different fractions of leaves, stem and fruit of
M. nigra, respectively. Good co-relation was found between TPCs and TEAC
values determined by ABTS•+ decolourization assay (r2 = 0.977, 0.943 and
0.823 for leaves, stem and the fruit of stem and FRAP assay (r2 = 0.887,
0.842 and 0.773 for leaves, stem and the fruit) of M. nigra. Employing total
antioxidant assay using linoleic acid emulsion system, methanol, 1-butanol,
aqueous and ethyl acetate fractions of all the parts of M. nigra showed strong
peroxyl radicals scavenging activity. The data obtained by employing antioxidant
and radical scavenging assays demonstrated the effectiveness of all
the parts of M. nigra as antioxidative and radical scavenging which may also
be correlated with its anti-disease activity in in vivo.
antioxidants. Morus nigra L. is considered to be an antidiabetic and diuretic
agent in the folk medicinal system of Indo-Pak subcontinent. Antioxidant
potential and radical scavenging activity of the various organic and aqueous
extracts of leaves, stem and fruit of M. nigra were investigated using 2,2'-
azinobis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) decolourization
assay, ferric reducing antioxidant power (FRAP) assay, 2.2'-diphenyl-1-picrylhydrazyl
(DPPH) assay, total phenolic content (TPC) assay and total antioxidant
activity assay. Using ABTS•+ decolourization assay and FRAP assay, M.
nigra extracts showed a wide range of antioxidant activity. Using TPC assay
the amount of total phenolics ranged from 1.3048 to 5.8287, 0.413 to 2.9394
and 0.5346 to 6.2266 mg/L for different fractions of leaves, stem and fruit of
M. nigra, respectively. Good co-relation was found between TPCs and TEAC
values determined by ABTS•+ decolourization assay (r2 = 0.977, 0.943 and
0.823 for leaves, stem and the fruit of stem and FRAP assay (r2 = 0.887,
0.842 and 0.773 for leaves, stem and the fruit) of M. nigra. Employing total
antioxidant assay using linoleic acid emulsion system, methanol, 1-butanol,
aqueous and ethyl acetate fractions of all the parts of M. nigra showed strong
peroxyl radicals scavenging activity. The data obtained by employing antioxidant
and radical scavenging assays demonstrated the effectiveness of all
the parts of M. nigra as antioxidative and radical scavenging which may also
be correlated with its anti-disease activity in in vivo.
Keywords
Antioxidant potential
Radical scavenging
TEAC
Morus nigra.
IQBAL, S., NADEEM ASGHAR*†, M., ULLAH KHAN, I., & ZIA, I. (2009). Antioxidant Potential Profile of Extracts from Different Parts of Black Mulberry. Asian Journal of Chemistry, 22(1), 353–364. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/16655
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