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Simple, Rapid and Sensitive UV Spectrophotometric Method for Determination of Temozolomide in Poly-ε-caprolactone Nanoparticles
Corresponding Author(s) : Archana Khosa
Asian Journal of Chemistry,
Vol. 30 No. 4 (2018): Vol 30 Issue 4
Abstract
The objective of this work was to develop and validate a simple, rapid and cost effective UV spectrophotometric method for pharmaceutical characterization of temozolomide loaded polymeric nanoparticles. The media selected was 100 mM hydrochloric acid medium (pH 1.2) and λmax of temozolomide was detected at 329 nm. The method was shown to be specific, linear in the range of 3-25 μg mL-1 (R2 = 0.999), accurate (recovery ranging from 99.34 to 100.90 %), precise at the intra-day and inter-day levels as reflected by the relative standard deviation values of less than 1.28 % and robust to changes in diluent media pH. The detection and quantitation limits were 0.170 and 0.510 μg mL–1 respectively indicating sensitivity of the method. The method was successfully used to determine the drug content and entrapment efficiency of temozolomide loaded in poly-ε-caprolactone nanoparticles.
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- S.K. Chowdhury, D. Laudicina, N. Blumenkrantz, M. Wirth and K.B. Alton, J. Pharm. Biomed. Anal., 19, 659 (1999); https://doi.org/10.1016/S0731-7085(98)00198-8.
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- A.L. Rao, G.T. Ramesh and S. Rao, Asian J. Chem., 22, 5067 (2010).
- A.A. Razak, S. Masthanamma, B. Omshanthi, V. Suresh and P. Obulamma, Int. J. Pharm. Sci. Res., 4, 1419 (2013).
- B.M. Ishaq, H.A. Ahad, S. Muneer, S. Parveen and B. Fahmida, Int. Res. J. Pharm., 5, 17 (2014).
- ICH Guideline, Validation of Analytical Procedures: Text and Methodology, Q2 (R1) 1 (2005).
- E.S. Newlands, M.F.G. Stevens, S.R. Wedge, R.T. Wheelhouse and C. Brock, Cancer Treat. Rev., 23, 35 (1997); https://doi.org/10.1016/S0305-7372(97)90019-0.
References
S.K. Chowdhury, D. Laudicina, N. Blumenkrantz, M. Wirth and K.B. Alton, J. Pharm. Biomed. Anal., 19, 659 (1999); https://doi.org/10.1016/S0731-7085(98)00198-8.
H.J. Meany, K.E. Warren, E. Fox, D.E. Cole, A.A. Aikin and F.M. Balis, Cancer Chemother. Pharmacol., 65, 137 (2009); https://doi.org/10.1007/s00280-009-1015-8.
M. Andrasi, R. Bustos, A. Gaspar, F.A. Gomez and A. Klekner, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 878, 1801 (2010); https://doi.org/10.1016/j.jchromb.2010.05.008.
F. Shen, L.A. Decosterd, M. Gander, S. Leyvraz, J. Biollaz and F. Lejeune, J. Chromatogr. B Biomed. Sci. Appl., 667, 291 (1995); https://doi.org/10.1016/0378-4347(95)00040-P.
S. Ostermann, C. Csajka, T. Buclin, S. Leyvraz, F. Lejeune, L.A. Decosterd and R. Stupp, Clin. Cancer Res., 10, 3728 (2004); https://doi.org/10.1158/1078-0432.CCR-03-0807.
H. Kim, P. Likhari, D. Parker, P. Statkevich, A. Marco, C. Lin and A.A. Nomeir, J. Pharm. Biomed. Anal., 24, 461 (2001); https://doi.org/10.1016/S0731-7085(00)00466-0.
L. Reyderman, P. Statkevich, C.M. Thonoor, J. Patrick, V.K. Batra and M. Wirth, Xenobiotica, 34, 487 (2004); https://doi.org/10.1080/00498250410001685737.
A. Khan, S.S. Imam, M. Aqil, Y. Sultana, A. Ali and K. Khan, Beni-Suef Univ. J. Basic Appl. Sci., 5, 402 (2016); https://doi.org/10.1016/j.bjbas.2015.11.011.
A.L. Rao, G.T. Ramesh and S. Rao, Asian J. Chem., 22, 5067 (2010).
A.A. Razak, S. Masthanamma, B. Omshanthi, V. Suresh and P. Obulamma, Int. J. Pharm. Sci. Res., 4, 1419 (2013).
B.M. Ishaq, H.A. Ahad, S. Muneer, S. Parveen and B. Fahmida, Int. Res. J. Pharm., 5, 17 (2014).
ICH Guideline, Validation of Analytical Procedures: Text and Methodology, Q2 (R1) 1 (2005).
E.S. Newlands, M.F.G. Stevens, S.R. Wedge, R.T. Wheelhouse and C. Brock, Cancer Treat. Rev., 23, 35 (1997); https://doi.org/10.1016/S0305-7372(97)90019-0.