Isolation and Identification of Iridoid from Water Extract of Leaves of Vitex pinnata Linn. as Antioxidant

Mastura; Tonel Barus; Lamek Marpaung; Partomuan Simanjuntak

doi:10.14233/ajchem.2018.21048

Issue

Vol. 30 No. 4 (2018): Vol 30 Issue 4

Issue Published : February 28, 2018

This work is licensed under a Creative Commons Attribution 4.0 International License.

Isolation and Identification of Iridoid from Water Extract of Leaves of Vitex pinnata Linn. as Antioxidant

https://doi.org/10.14233/ajchem.2018.21048

Mastura

Samudra University, Meurandeh, Kota Langsa 24416, Aceh, Indonesia; Sumatera Utara University, Medan, Indonesia

Tonel Barus

Sumatera Utara University, Medan, Indonesia

Lamek Marpaung

Sumatera Utara University, Medan, Indonesia

Partomuan Simanjuntak

Research Center for Biotechnology, Indonesian Institute of Sciences (LIPI), Jl. Raya Bogor Km. 46, Cibinong 16911, Indonesia

Corresponding Author(s) : Mastura

mastura_fkipunsam@yahoo.co.id

Asian Journal of Chemistry, Vol. 30 No. 4 (2018): Vol 30 Issue 4
Article Published : February 28, 2018

Abstract

An iridoid compound with antioxidant activity was isolated from the water extract of leaves of Halban (Vitex pinnata Linn.). Isolation and identification processes of the compound were performed based on the bioassay-activity-guided method using the free radical scavenging method for antioxidant. The purification was done by the column chromatography analysis [(SiO₂; (i) dichloromethane:methanol = 5:1–1:1, (ii) dichloromethane:methanol:water = 5:5:1, (iii) dichloromethane:methanol = 5:1)] to give white powder. Chemical structure determination was done by an interpretation of spectroscopy data (UV, IR, NMR and MS) and revealed a glycoside iridoid compound with the high antioxidant activity (IC₅₀ 27.3 ppm).

Keywords

Vitex pinnata Linn. Glycoside iridoid Antioxidant

Mastura, ., Barus, T., Marpaung, L., & Simanjuntak, P. (2018). Isolation and Identification of Iridoid from Water Extract of Leaves of Vitex pinnata Linn. as Antioxidant. Asian Journal of Chemistry, 30(4), 814–816. https://doi.org/10.14233/ajchem.2018.21048

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References

I.W. Kusuma, Murdiyanto, E.T. Arung, Syafrizal and Y. Kim, Food Sci. Human Wellness, 3, 191 (2014); https://doi.org/10.1016/j.fshw.2014.12.004.
Y. Ogata, Y. Kasahara and T. Iwasaki, Medicine Herb Index Indonesia, Eisai Indonesia, edn 2 (1995).
I.H. Burkill, A Dictionary of Economic Products of The Malay Peninsula, Ministry of Agriculture and Cooperative, Kuala Lumpur, Vol. II (1966).
Syafruddin, Isolasi dan Identifikasi Senyawa Flavonoid dari Buah Laban (Vitex pinnata Linn.) Asal Nanggroe Aceh Darussalam, Serambi Saintia: Vol. 1, No. 1 (2013) (In Indonesian).
L.A. del Río, J. Exp. Bot., 66, 2827 (2015); https://doi.org/10.1093/jxb/erv099.
S. Mahmoudi, M. Khali, A. Benkhaled, K. Benamirouche and I. Baiti, Asian Pac. J. Trop. Biomed., 6, 239 (2016); https://doi.org/10.1016/j.apjtb.2015.12.010.
S. Mondal, R.R. Phadke and A.M. Badigannavar, Euphytica, 204, 311 (2015); https://doi.org/10.1007/s10681-014-1324-9.
P. Molyneux, Songklanakarin J. Sci. Technol., 26, 211 (2004).
M.R. Boyd, ed. B.A. Teicher, The NCI in vitro Anticancer Drug Discovery Screen: Concept, Implementation and Operation (1985–1995), In: Anticancer Drug Development Guide, Preclinical Screening, Clinical Trials and Approval, Humana Press, Totowa, pp. 23-42 (1997).
E. Pretsch, P. Bühlmann and C. Affolter, Structure Determination of Organic Compounds, Springer-Verlag Berlin Heidelberg (2009).
R.M. Silverstein, G.C. Basler and T.C. Morrill, Spectroscopic Identification of Organic Compounds, John Wiley & Sons Publ. (1986).
L.J. El-Naggar and J.L. Beal, J. Nat. Prod., 43, 649 (1980); https://doi.org/10.1021/np50012a001.
M. Reichenbächer and J. Popp, Challenges in Molecular Structure Determination, Springer-Verlag, Berlin Heidelberg (2012).
S. Roy, K. Bairwa, J. Grover, A. Srivastava and S.M. Jachak, J. Anal. Chem., 70, 639 (2015); https://doi.org/10.1134/S1061934815050147.
S. Shah, T. Dhanani and S. Kumar, J. Pharm. Anal., 3, 500 (2013); https://doi.org/10.1016/j.jpha.2013.09.008.

References

I.W. Kusuma, Murdiyanto, E.T. Arung, Syafrizal and Y. Kim, Food Sci. Human Wellness, 3, 191 (2014); https://doi.org/10.1016/j.fshw.2014.12.004.

Y. Ogata, Y. Kasahara and T. Iwasaki, Medicine Herb Index Indonesia, Eisai Indonesia, edn 2 (1995).

I.H. Burkill, A Dictionary of Economic Products of The Malay Peninsula, Ministry of Agriculture and Cooperative, Kuala Lumpur, Vol. II (1966).

Syafruddin, Isolasi dan Identifikasi Senyawa Flavonoid dari Buah Laban (Vitex pinnata Linn.) Asal Nanggroe Aceh Darussalam, Serambi Saintia: Vol. 1, No. 1 (2013) (In Indonesian).

L.A. del Río, J. Exp. Bot., 66, 2827 (2015); https://doi.org/10.1093/jxb/erv099.

S. Mahmoudi, M. Khali, A. Benkhaled, K. Benamirouche and I. Baiti, Asian Pac. J. Trop. Biomed., 6, 239 (2016); https://doi.org/10.1016/j.apjtb.2015.12.010.

S. Mondal, R.R. Phadke and A.M. Badigannavar, Euphytica, 204, 311 (2015); https://doi.org/10.1007/s10681-014-1324-9.

P. Molyneux, Songklanakarin J. Sci. Technol., 26, 211 (2004).

M.R. Boyd, ed. B.A. Teicher, The NCI in vitro Anticancer Drug Discovery Screen: Concept, Implementation and Operation (1985–1995), In: Anticancer Drug Development Guide, Preclinical Screening, Clinical Trials and Approval, Humana Press, Totowa, pp. 23-42 (1997).

E. Pretsch, P. Bühlmann and C. Affolter, Structure Determination of Organic Compounds, Springer-Verlag Berlin Heidelberg (2009).

R.M. Silverstein, G.C. Basler and T.C. Morrill, Spectroscopic Identification of Organic Compounds, John Wiley & Sons Publ. (1986).

L.J. El-Naggar and J.L. Beal, J. Nat. Prod., 43, 649 (1980); https://doi.org/10.1021/np50012a001.

M. Reichenbächer and J. Popp, Challenges in Molecular Structure Determination, Springer-Verlag, Berlin Heidelberg (2012).

S. Roy, K. Bairwa, J. Grover, A. Srivastava and S.M. Jachak, J. Anal. Chem., 70, 639 (2015); https://doi.org/10.1134/S1061934815050147.

S. Shah, T. Dhanani and S. Kumar, J. Pharm. Anal., 3, 500 (2013); https://doi.org/10.1016/j.jpha.2013.09.008.

Issue

Vol. 30 No. 4 (2018): Vol 30 Issue 4

Isolation and Identification of Iridoid from Water Extract of Leaves of Vitex pinnata Linn. as Antioxidant

Corresponding Author(s) : Mastura

Abstract

Keywords

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