H-Point Standard Addition Method for Simultaneous Spectrophotometric Determination of Hydrochlorothiazide and Furosemide

Abstract

The applicability of H-point standard addition method for resolving of overlapping spectra corresponding to the hydrochlorothiazide and furosemide is verified. The results show that the H-point standard addition method is suitable for the simultaneous determination of hydrochlorothiazide and furosemide in aqueous media. The results of applying the H-point standard addition method showed that the two drugs could be determined simultaneously with the concentration ratios of hydrochlorothiazide to furosemide varying from 1:40-20:1 in the mixed sample. The proposed method has been successfully applied to the simultaneous determination of hydrochlorothiazide in the presence of furosemide in some synthetic samples. Moreover, the applicability of the method was demonstrated by the recovery of species in some biological fluids.