RP-HPLC Determination of Hydrocortisone in Parenterals

Abstract

A precise reverse phase HPLC method has been developed for the determination of hydrocortisone in parenteral dosage forms. The chromatography was carried out on an ODS column using a mixture of phosphoric acid, acetonitrile and water (0.5:380:620 v/v) as the mobile phase at a flow rate of 1.0 mL/min. The detection of the drug in the eluates was done at 245 nm. The retention time obtained for the drug was 4.38 min. The method produced linear responses in the concentration range of 2-20 μg/mL of hydrocortisone. The method was also found to be applicable for determination of the drug in parenteral preparations with an average per cent recovery of 99.83.