RP-HPLC Estimation of Valacyclovir HCl in Tablet Formulation

Abstract

A simple, rapid, sensitive and fully validated reverse phase HPLC method has been described for estimation of valacyclovir in tablet dosage form using mobile phase without any column degrading agent. Here in present method, chromatography was achieved on an ODS column, 0.015 M acetic acid and methanol (95:5 v/v) as the mobile phase with a flow rate of 1.1 mL/min and 254 nm as wavelength for monitoring eluent. The retention time of the drug was 3.0 min. The detector response was linear in the concentration of 6-90 μg/mL with correlation coefficient 0.999. The limit of detection (LOD) and limit of quantification (LOQ) were 3.4 and 4.6 μg/mL, respectively. The percentage assay of valacyclovir hydrochloride was 99.861-100.45 %. The method was validated by determining its sensitivity, accuracy and precision. This method is suitable not only on the parameters validated but also the mobile phase utilized which consists of only methanol and acetic acid both of them were not affects the column life and performance.