Issue

Vol. 30 No. 3 (2018): Vol 30 Issue 3

Copyright (c) 2018 AJC

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Design, Synthesis and Characterization of Biogenic Chloroquine Silver Nanoparticles as Potential Anticancer Agent against Neuroblastoma Cells

https://doi.org/10.14233/ajchem.2018.20952
S.C. Vivekanandhan
Department of Medicine, Thoothukudi Medical College, Thoothukudi-628 008, India
M. Chandramohan
Bharat Ratna Kamarajar Jaundice Liver Hospital and Research Centre, Madurai-625 001, India
P. Selvam
Sir CV Raman-K.S. Krishnan International Research Centre, Kalasalingam University, Krishnankoil-626 126, India

Corresponding Author(s) : P. Selvam

selvaamin@gmail.com

Asian Journal of Chemistry, Vol. 30 No. 3 (2018): Vol 30 Issue 3

Abstract

Chloroquine (4-aminoquinoline derivative) an old antimalarial drug is a versatile bioactive agent and reported to have board spectrum of activity including anticancer and antiviral activity and firmly repositioned in these latter indications. In literature report of chloroquine retinopathy upto 0.5 % was noted to circumvent this toxicity and increase the efficacy of the drug. We ventured to develop a novel approach of the green synthesis of chloroquine silver nanoparticles and anticancer activity of the same was tested by MTT against Neuroblastoma (SH-SY5Yc) and cytotoxicity was also investigated against normal Vero cells by MTT assay. We prepared chloroquine silver nanoparticles by an ecofriendly method and was characterized using FT-IR, UV-visible, SEM and PXRD techniques. Anticancer activity of chloroquine silver nanoparticles was tested against Neuroblastoma (SH-SY5Yc) by MTT assay and cytotoxicity in vero cells. The UV-visible spectrum for prepared silver nanoparticles shows sharp peak at 434 nm confirms the presence of silver nanoparticles. SEM analysis showed that the nanoparticles are spherical in shape and average size of the particles are 254 nm. Powder XRD studies, 2q value (220), (111), (211) confirms that the biosynthesized nanoparticles are in crystalline nature. The prepared chloroquine silver nanoparticles showed significant anticancer activity against Neuroblastoma cells by MTT assay with inhibitory concentration (IC50) of 33.79 μg/mL, where as cytotoxicity (CC50) of chloroquine silver nanoparticles in Vero cells was found to be 173.79 μg/mL.

Keywords

Chloroquine Silver nanoparticles Anticancer Activity Neuroblastoma cells
Vivekanandhan, S., Chandramohan, M., & Selvam, P. (2018). Design, Synthesis and Characterization of Biogenic Chloroquine Silver Nanoparticles as Potential Anticancer Agent against Neuroblastoma Cells. Asian Journal of Chemistry, 30(3), 537–540. https://doi.org/10.14233/ajchem.2018.20952
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. J.M. Rolain, P. Colson and D. Raoult, Int. J. Antimicrob. Agents, 30, 297 (2007); https://doi.org/10.1016/j.ijantimicag.2007.05.015.
  2. M.R. Fesen, K.W. Kohn, F. Leteurtre and Y. Pommier, Proc. Natl. Acad. Sci. USA, 90, 2399 (1993); https://doi.org/10.1073/pnas.90.6.2399.
  3. G. Civitico, Y.Y. Wang, C. Luscombe, N. Bishop, G. Tachedjian, I. Gust and S. Locarnini, J. Med. Virol., 31, 90 (1990); https://doi.org/10.1002/jmv.1890310205.
  4. M. Chandramohan, S.C. Vivekananthan, D. Sivakumar, P. Selvam, J. Neyts, G. Katrien and E. De Clercq, Indian J. Pharm. Sci., 68, 538 (2006); https://doi.org/10.4103/0250-474X.27842.
  5. U.A. Ashfaq, T. Javed, S. Rehman, Z. Nawaz and S. Riazuddin, Virol. J., 8, 163 (2011); https://doi.org/10.1186/1743-422X-8-163.
  6. N.I. Paton, L. Lee, Y. Xu, E.E. Ooi, Y.B. Cheung, S. Archuleta, G. Wong and A.W. Smith, Lancet Infect. Dis., 11, 677 (2011); https://doi.org/10.1016/S1473-3099(11)70065-2.
  7. K.J. Farias, P.R. Machado, J.A. Muniz, A.A. Imbeloni and B.A. Da Fonseca, Viral Immunol., 28, 161 (2015); https://doi.org/10.1089/vim.2014.0090.
  8. E. Keyaerts, L. Vijgen, P. Maes, J. Neyts and M.V. Ranst, Biochem. Biophys. Res. Commun., 323, 264 (2004); https://doi.org/10.1016/j.bbrc.2004.08.085.
  9. M. Khan, S.R. Santhosh, M. Tiwari, P.V. Lakshmana Rao and M. Parida, J. Med. Virol., 82, 817 (2010); https://doi.org/10.1002/jmv.21663.
  10. S. Hatziieremia, P. McCall, J. Willder, C. Orange, M. Seywright, M.A. Underwood and J. Edwards, Cancer Res., 73, 1 (2013); https://doi.org/10.1158/1538-7445.AM2013-1.
  11. M. Gwenola, O. Florine, K. Guido, V. Illio and G. Lorenzo, Mol. Cel. Oncol., e29911 (2014); https://doi.org/10.4161/mco.29911.
  12. V. Solomon and H. Lee, Eur. J. Pharmacol., 625, 220 (2009); https://doi.org/10.1016/j.ejphar.2009.06.063.
  13. Y.C. Lin, J.F. Lin, S.I. Wen, S.C. Yang, T.F. Tsai, H.E. Chen, K.Y. Chou and T.I. Hwang, Kaohsiung J. Med. Sci., 33, 215 (2017); https://doi.org/10.1016/j.kjms.2017.01.004.
  14. M.E. Egger, J.S. Huang, W. Yin, K.M. McMasters and L.R. McNally, J. Surg. Res., 184, 274 (2013); https://doi.org/10.1016/j.jss.2013.04.055.
  15. H. Zhao, Y. Liao, D. Xu, Q. Wang, Q. Gan, C. You and C. Yang, BMC Neurol., 16, 1 (2016); https://doi.org/10.1186/s12883-015-0524-9.
  16. M.F. Marmor, U. Kellner, T.Y. Lai, R.B. Melles and W.F. Mieler, Am. Acad. Ophthalmol., 123, 1386 (2016); https://doi.org/10.1016/j.ophtha.2016.01.058.
  17. L.S. Leung, J.W. Neal, H.A. Wakelee, L.V. Sequist and M.F. Marmor, Am. J. Ophthalmol., 160, 799 (2015); https://doi.org/10.1016/j.ajo.2015.07.012.
  18. E.V. Navajas, H. Krema, D.S. Hammoudi, J.H. Lipton, E.R. Simpson, S. Boyd and M. Easterbrook, Can. J. Ophthalmol., 50, 442 (2015); https://doi.org/10.1016/j.jcjo.2015.08.003.
  19. P. Skehan, R. Storeng, D. Scudiero, A. Monks, J. McMahon, D. Vistica, J.T. Warren, H. Bokesch, S. Kenney and M.R. Boyd, J. Natl. Cancer Inst., 82, 1107 (1990); https://doi.org/10.1093/jnci/82.13.1107.
  20. P. Joshi, S. Chakraborty, S. Dey, V. Shanker, Z.A. Ansari, S.P. Singh and P. Chakrabarti, J. Colloid Interf. Sci., 355, 402 (2011); https://doi.org/10.1016/j.jcis.2010.12.032.
  21. P. Joshi, S. Chakraborti, J.E. Ramirez-Vick, Z.A. Ansari, P. Chakraborti and S.P. Singh, Colloids Surf. B Biointerf., 15, 195 (2012); https://doi.org/10.1016/j.colsurfb.2012.02.039.
  22. S. Tripathy, S. Das, S.P. Chakraborty, S.K. Sahu, P. Pramanik and S. Roy, Int. J. Pharm., 434, 292 (2012); https://doi.org/10.1016/j.ijpharm.2012.05.064.
  23. K. Murugan, J. Wei, M.S. Alsalhi, M. Nicoletti, M. Paulpandi, C.M. Samidoss, D. Dinesh, B. Chandramohan, C. Paneerselvam, J. Subramaniam, H. Wei, C. Vadivalagan, P. Amuthavalli, A. Jaganathan, S. Devanesan, A. Higuchi, S. Kumar, A.T. Aziz, D. Nataraj, B. Vaseeharan, A. Canale and G. Benelli, Parasitol. Res., 116, 495 (2017); https://doi.org/10.1007/s00436-016-5310-0.
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 1 Download : 1