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Vol. 30 No. 3 (2018): Vol 30 Issue 3

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Cytotoxic Prenylflavanones from Philippine Stingless Bee (Tetragonula biroi Friese) Nests

https://doi.org/10.14233/ajchem.2018.21053
Mariquit M. De Los Reyes
Department of Biology, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines; Department of Biology, De La Salle University Manila - Laguna Campus, Biñan City, Laguna 4024, Philippines
Glenn G. Oyong
Department of Biology, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines; Center for Natural Science and Environmental Research, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines
Virgilio D. Ebajo Jr.
Department of Chemistry, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines
Chien-Chang Shen
National Research Institute of Chinese Medicine, Ministry of Health and Welfare, 155-1, Li-Nong St., Sec. 2, Taipei 112, Taiwan
Consolacion Y. Ragasa
Department of Chemistry, De La Salle University, 2401 Taft Avenue, Manila 1004, Philippines; Department of Chemistry, De La Salle University Manila - Laguna Campus, Biñan City, Laguna 4024, Philippines

Corresponding Author(s) : Mariquit M. De Los Reyes

mariquit.delosreyes@dlsu.edu.ph

Asian Journal of Chemistry, Vol. 30 No. 3 (2018): Vol 30 Issue 3

Abstract

The dichloromethane crude extract and compounds, glyasperin A (1), propolin E (2) and propolin A (3), obtained from Philippine stingless bee (Tetragonula biroi Friese) nests, were evaluated for their cytotoxic potentials. The anti-proliferative activities of crude extract and compounds 1-3 against human cancer cell lines, breast (MCF-7) and colon (HT-29 and HCT-116), and a normal cell line, human dermal fibroblast neonatal (HDFn), were evaluated using the PrestoBlue® cell viability assay. The crude extract was most anti-proliferative against HCT-116 cells (IC50 = 1.410 μg/mL). Glyasperin A (1) exhibited the strongest effect on MCF-7 cells (2.378 μg/mL). Propolin E (2) was most cytotoxic against HCT-116 cells (2.279 μg/mL), while propolin A (3) was most inhibitory against MCF-7 cells (2.815 μg/mL). Comparing the colorectal cancer cell lines, HCT-116 was generally more susceptible under crude extract, 2, and 3 (IC50 = 1.410, 2.279, 3.013 μg/mL, respectively) than HT-29 (5.620, 5.320, 5.359). Comparing the activities of propolin E and propolin A against HCT-116 cells, 2 (IC50 = 2.279 μg/mL) was more cytotoxic than 3 (3.013). The crude extract was more cytotoxic than Zeocin against HCT-116 cells. To our best of knowledge, this is the first report on the anti-proliferative properties of crude extract and compounds from T. biroi.

Keywords

Cytotoxicity Glyasperin A Philippine stingless bee Propolin A Propolin E Tetragonula biroi Friese
Los Reyes, M. M. D., Oyong, G. G., Ebajo Jr., V. D., Shen, C.-C., & Ragasa, C. Y. (2018). Cytotoxic Prenylflavanones from Philippine Stingless Bee (Tetragonula biroi Friese) Nests. Asian Journal of Chemistry, 30(3), 613–619. https://doi.org/10.14233/ajchem.2018.21053
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