Screening, Identification and Controlling Effect of Antifungal Compound from Bacillus megaterium LB01-16

Luo-Qing Wei; Cong-Wen Ding; Shang-Ze Zhou

doi:10.14233/ajchem.2020.22942

Issue

Vol. 32 No. 12 (2020): Vol 32 Issue 12, 2020

Issue Published : December 7, 2020

This work is licensed under a Creative Commons Attribution 4.0 International License.

Screening, Identification and Controlling Effect of Antifungal Compound from Bacillus megaterium LB01-16

https://doi.org/10.14233/ajchem.2020.22942

Luo-Qing Wei

College of Chemistry and Environment Engineering, Baise University, Baise 533000, Guangxi Province, P.R. China

Cong-Wen Ding

College of Chemistry and Environment Engineering, Baise University, Baise 533000, Guangxi Province, P.R. China

Shang-Ze Zhou

College of Chemistry and Environment Engineering, Baise University, Baise 533000, Guangxi Province, P.R. China

Corresponding Author(s) : Cong-Wen Ding

dingcw19731008@163.com

Asian Journal of Chemistry, Vol. 32 No. 12 (2020): Vol 32 Issue 12, 2020
Article Published : December 7, 2020

Abstract

In order to screen, identify high active compound and investigate its controlling effect on anthracnose disease in postharvest mangos, the bioassay-guided fractionation was used to screen and isolate antifungal components from fermented broth of Bacillus megaterium LB01-16 and the inhibition zone diameter of active components isolated against Colletotrichum gloeosporioides were measured with a disk diffusion test. The results revealed that four monomer compounds were separated from the fermentation broth, and only one monomer with strong antifungal activity, numbered 007, was obtained and identified as glucopyranosyl 12-hydroxyjasmonic acid by ¹H & ¹³C NMR and by comparing with literature. This study confirmed that the glucopyranosyl 12-hydroxyjasmonic acid produced from Bacillus megaterium LB01-16 can inhibit the anthracnose disease of postharvest mangos by directly reducing mycelium growth and inhibiting the spore germination of Colletotrichum gloeosporioides, thus providing a promising strategy for controlling of anthracnose disease in postharvest mangos.

Keywords

Bacillus megaterium Anthracnose disease Postharvest mangos Colletotrichum gloeosporioides Controlling effect

Wei, L.-Q., Ding, C.-W., & Zhou, S.-Z. (2020). Screening, Identification and Controlling Effect of Antifungal Compound from Bacillus megaterium LB01-16. Asian Journal of Chemistry, 32(12), 3063–3066. https://doi.org/10.14233/ajchem.2020.22942

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Z. Chen, J. Huang and J. Zhao, Biotech. Bull., 33, 81 (2017).

Q. Kong, C. Chi, S.H. Shan, Q.Y. Li and J. Zhejiang Univ, Agric. Life Sci. Ed., 41, 567 (2015).

Y.R. Wang, J.S. Zhang and J.L. Li, China Food Addit., 8, 63 (2017).

K. Hayakawa, M. Kimura, K. Kasaha, K. Matsumoto, H. Sansawa and Y. Yamori, Br. J. Nutr., 92, 411 (2004); https://doi.org/10.1079/BJN20041221

K. Inoue, T. Shirai, H. Ochiai, M. Kasao, K. Hayakawa, M. Kimura and H. Sansawa, Eur. J. Clin. Nutr., 57, 490 (2003); https://doi.org/10.1038/sj.ejcn.1601555

J.Y. Zhao, Y. Xiao, Y.Y. Ji and Z. Li, Shipin Kexue, 40, 1 (2019).

C.W. Ding, Q. Feng and C.H. Li, Sci. Technol. Food Ind., 41, 131(2020).

A. Ghasemi Pirbalouti, S.E. Sajjadi and K. Parang, Arch. Pharm., 347, 229 (2014); https://doi.org/10.1002/ardp.201300287

L. Fu, S.Y. Liu and F.X. Xu, J. Shenyang Normal Univ., 35, 485 (2017).

Y. Ji, J. Li and L. Wang, Shipin Kexue, 39, 258 (2018).

S. Wang, H. Takahashi, T. Saito, K. Okawa, H. Ohara, M. Shishido, H. Ikeura and S. Kondo, Sci. Hortic., 192, 166 (2015); https://doi.org/10.1016/j.scienta.2015.06.001

X.X. Li, Y.H. Long and X.H. Yin, Shipin Kexue, 40, 239 (2019).

G.Y. Li, Y.H. Ge and D.S. Zhu, Shipin Kexue, 36, 255 (2015).

R. Rotem, A. Heyfets, O. Fingrut, D. Blickstein, M. Shaklai and E. Flescher, Cancer Res., 65, 1984 (2005); https://doi.org/10.1158/0008-5472.CAN-04-3091

A. Chatzopoulou, A. Karioti, C. Gousiadou, V.L. Vivancos, S. Golegou, P. Kyriazopoulos and H. Skaltsa, J. Agric. Food Chem., 58, 6064 (2010); https://doi.org/10.1021/jf904596m

WJ. Hurst, D.A. Stuart, A.I.C. Justavino and R.B. van Breemen, Jasmonic Acid Compounds in Cocoa Products, US Patent US9040096B2 (2011).

F. Flescher, Y. Kashman, D. Reischer and S. Shimony, Jasmonate Derivative Compounds Pharmaceutical Compounds and Methods of Use Thereof, US Patent US7683211B2 (2010).

E. Flescher, N. Goldin and M. Herzberg, Jasmonate Based Assays for Identifying Compounds that Selectively Inhibit Mitochondrial Bound Hexokinases, US Patent US8349553B2 (2013).

V. Pøikrylová, M. Beran, P. Sedmera and J. Jizba, Folia Microbiol., 39, 191(1994); https://doi.org/10.1007/BF02814647

K. Kurosawa, K. Takahashi and E. Tsuda, J. Antibiot., 54, 615 (2001); https://doi.org/10.7164/antibiotics.54.615

C.Y. Cen, Z.X. Huang, S.D. Li, J.G. Wei, L.B. Li and R.S. Huang, Agric. Sci., 55, 912 (2016).

M. Ueda, M. Okazaki, K. Ueda and S. Yamamura, Tetrahedron, 56, 8101 (2000); https://doi.org/10.1016/S0040-4020(00)00729-8

Issue

Vol. 32 No. 12 (2020): Vol 32 Issue 12, 2020

Screening, Identification and Controlling Effect of Antifungal Compound from Bacillus megaterium LB01-16

Corresponding Author(s) : Cong-Wen Ding

Abstract

Keywords

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