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Electrochemical and Spectroscopic Studies of Interaction of Clopidogrel Bisulphate with Calf Thymus DNA

https://doi.org/10.14233/ajchem.2018.20932
S. Malini
Department of Chemistry, Dayananda Sagar College of Engineering, Kumaraswamy Layout, Bangalore-560 078, India
Kalyan Raj
Department of Chemistry, B.M.S. College of Engineering, Basavanagudi, Bangalore-560 009, India
M. Sennappan
Department of Chemistry, Dayananda Sagar College of Engineering, Kumaraswamy Layout, Bangalore-560 078, India

Corresponding Author(s) : S. Malini

malini.chandrashekhar@gmail.com

Asian Journal of Chemistry, Vol. 30 No. 1 (2018): Vol 30 Issue 1

Abstract

The DNA binding properties of clopidogrel bisulphate, was examined by cyclic voltammetry, fluorescence and UV spectroscopy. Shift in peak potential, decrease in peak current in cyclic voltammetry, hypochromic effect in UV titration and quenched emission intensity of ethidium bromide bound to calf thymus DNA signal indicated clopidogrel bisulphate has displaced ethidium bromide from its binding site in DNA. The binding constant is calculated using absorbance and is found to be closer to that estimated in voltammetric measurements. The results also corroborated well with fluorescence measurements and represent that the binding proceeds by intercalation.

Keywords

Clopidogrel bisulphate DNA binding Cyclic voltammetry Spectrophotometry
Malini, S., Raj, K., & Sennappan, M. (2017). Electrochemical and Spectroscopic Studies of Interaction of Clopidogrel Bisulphate with Calf Thymus DNA. Asian Journal of Chemistry, 30(1), 129–132. https://doi.org/10.14233/ajchem.2018.20932
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