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Isolation and Identification of Pure Bioactive Compounds from Cuscuta reflexa Grown on Nerium oleander Host Plant by Flash Column Chromatography and GC-MS/MS Analysis
Corresponding Author(s) : Pooja Saini
Asian Journal of Chemistry,
Vol. 32 No. 10 (2020): Vol 32 Issue 10
Abstract
In this work, an ethyl acetate extract of Cuscuta reflexa grown on Nerium oleander host plant was used for the isolation and identification of pure bioactive compounds. Chloroform:methanol was used as mobile phase for flash column chromatography. Five pure bioactive compounds were isolated and two compounds were identified using GC-MS/MS technique. Glycerol-1-palmitate and squalene were identified in Cuscuta reflexa using NIST library on the basis of area percentage.
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- D. Savoia, Future Microbiol., 7, 979 (2012); https://doi.org/10.2217/fmb.12.68
- N.R. Farnsworth eds.: S. Larsen, S. Christensen and H. Kofod, The Role of Medicinal Plants in Drug Development, In: Natural Products and Drug Development, Munksgaard: Copenhagen, pp 34-45 (1994).
- K.H. Lee, J. Biomed. Sci., 6, 236 (1999); https://doi.org/10.1007/BF02253565
- S. Stefanovic and R.G. Olmstead, Syst. Biol., 53, 384 (2004); https://doi.org/10.1080/10635150490445896
- A. Ahmad, S. Tandon, T.D. Xuan and Z. Nooreen, Biomed. Pharmacother., 92, 772 (2017); https://doi.org/10.1016/j.biopha.2017.05.124
- Lalchand, R. Sahu, R. Gupta and O.P. Rout, Int. J. Res. Ayurveda Pharm., 8, 38 (2017); https://doi.org/10.7897/2277-4343.086288
- L.V. Costa-Lotufo, M.T.H. Khan, A. Ather, D.V. Wilke, P.C. Jimenez, C. Pessoa, M.E.A. de Moraes and M.O. de Moraes, J. Ethnopharmacol., 99, 21 (2005); https://doi.org/10.1016/j.jep.2005.01.041
- S. Khan, K.J. Mirza and M.Z. Abdin, Eur. Asia J. BioSci., 4, 1 (2010).
- U. Jha and T. Shelke, Int. J. Res. Ayurveda Pharm., 2, 1290 (2011).
- P. Solat, H.B. Iftikhar, U.A. Qurat, S. Kousar and J. Rehman, J. Appl. Sci., 3, 43 (2013).
- C. Dandopani, R. Sahu, A. Jha and J. Dwivedi, Trop. J. Pharm. Res., 10, 447 (2011).
- B. Ramya, E. Natrajan, S. Vijikumar, S. Muthukumarasamy and J. Vasanth, J. Nat. Sci., 1, 134 (2010).
- M.S. Siddiqui, A.A. Memon, S. Memon and S.G. Baloch, J. Herbs Spices Med. Plants, 23, 157 (2017); https://doi.org/10.1080/10496475.2017.1280867
- R.R. Kondeti, K.S. Mulpuri and B. Meruga, World J. Pharm. Sci., 2, 1375 (2014).
- C. Kaushal and B. Srivastava, J. Chem. Pharm. Res., 2, 519 (2010).
References
D. Savoia, Future Microbiol., 7, 979 (2012); https://doi.org/10.2217/fmb.12.68
N.R. Farnsworth eds.: S. Larsen, S. Christensen and H. Kofod, The Role of Medicinal Plants in Drug Development, In: Natural Products and Drug Development, Munksgaard: Copenhagen, pp 34-45 (1994).
K.H. Lee, J. Biomed. Sci., 6, 236 (1999); https://doi.org/10.1007/BF02253565
S. Stefanovic and R.G. Olmstead, Syst. Biol., 53, 384 (2004); https://doi.org/10.1080/10635150490445896
A. Ahmad, S. Tandon, T.D. Xuan and Z. Nooreen, Biomed. Pharmacother., 92, 772 (2017); https://doi.org/10.1016/j.biopha.2017.05.124
Lalchand, R. Sahu, R. Gupta and O.P. Rout, Int. J. Res. Ayurveda Pharm., 8, 38 (2017); https://doi.org/10.7897/2277-4343.086288
L.V. Costa-Lotufo, M.T.H. Khan, A. Ather, D.V. Wilke, P.C. Jimenez, C. Pessoa, M.E.A. de Moraes and M.O. de Moraes, J. Ethnopharmacol., 99, 21 (2005); https://doi.org/10.1016/j.jep.2005.01.041
S. Khan, K.J. Mirza and M.Z. Abdin, Eur. Asia J. BioSci., 4, 1 (2010).
U. Jha and T. Shelke, Int. J. Res. Ayurveda Pharm., 2, 1290 (2011).
P. Solat, H.B. Iftikhar, U.A. Qurat, S. Kousar and J. Rehman, J. Appl. Sci., 3, 43 (2013).
C. Dandopani, R. Sahu, A. Jha and J. Dwivedi, Trop. J. Pharm. Res., 10, 447 (2011).
B. Ramya, E. Natrajan, S. Vijikumar, S. Muthukumarasamy and J. Vasanth, J. Nat. Sci., 1, 134 (2010).
M.S. Siddiqui, A.A. Memon, S. Memon and S.G. Baloch, J. Herbs Spices Med. Plants, 23, 157 (2017); https://doi.org/10.1080/10496475.2017.1280867
R.R. Kondeti, K.S. Mulpuri and B. Meruga, World J. Pharm. Sci., 2, 1375 (2014).
C. Kaushal and B. Srivastava, J. Chem. Pharm. Res., 2, 519 (2010).