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Vol. 29 No. 5 (2017): Vol 29 Issue 5

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Simultaneous Estimation of Eprosartan and Hydrochlorthiazide in Pharmaceutical Dosage Forms by RP-HPLC

https://doi.org/10.14233/ajchem.2017.20419
Santoshi G. Rajamahanti
Department of Engineering Chemistry, AUCE (A), Andhra University, Visakhapatnam-530 003, India
N. Annapurna
Department of Engineering Chemistry, AUCE (A), Andhra University, Visakhapatnam-530 003, India
T. Santosh
Corpuscle Research Solutions, Visakhapatnam-530 017, India
B. Durga
Department of Engineering Chemistry, AUCE (A), Andhra University, Visakhapatnam-530 003, India
Sk. Raziya
Department of Engineering Chemistry, AUCE (A), Andhra University, Visakhapatnam-530 003, India

Corresponding Author(s) : Santoshi G. Rajamahanti

srisambhavi@gmail.com

Asian Journal of Chemistry, Vol. 29 No. 5 (2017): Vol 29 Issue 5

Abstract

An isocratic reverse phase high performance liquid chromatographic method was developed and validated for the simultaneous determination of eprosartan and hydrochlorothiazide in their binary mixtures of pharmaceutical dosage form preparation. The RP-HPLC method was performed by using a Agilent Polaris C18 column (150 × 4.6 mm id, ODS 2, 5 μm particle size) where the mobile phase was composed with 0.1 % orthophosphoric acid-methanol [(50:50 v/v) pH 3.10-0.04 adjusted with acetic acid] at a flow rate of 0.8 mL/min. The column temperature was maintained at 27 ± 5 °C and the detection was carried out by means of a UV detector at wavelength of 232 nm. The retention times of eprosartan and hydrochlorothiazide are 4.18 ± 0.10 and 2.53 ± 0.09 min, respectively. The method was found to be linear (R2 > 0.99) precise (RSD < 2.0 %), accurate, specific, simple, sensitive, rapid and robust. The validated method can be used in routine quality control analysis of fixed dose tablet formulations having eprosartan and hydrochlorothiazide without any interference of excipients.

Keywords

Eprosartan Hydrochlorothiazide RP-HPLC Tablet formulation
Rajamahanti, S. G., Annapurna, N., Santosh, T., Durga, B., & Raziya, S. (2017). Simultaneous Estimation of Eprosartan and Hydrochlorthiazide in Pharmaceutical Dosage Forms by RP-HPLC. Asian Journal of Chemistry, 29(5), 1091–1094. https://doi.org/10.14233/ajchem.2017.20419
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References
  1. J.P. Yaxley and S.V. Thambar, J. Family Med. Prim. Care, 4, 193 (2015); https://doi.org/10.4103/2249-4863.154630.
  2. S.N. Covey, Trends Pharmacol. Sci., 5, 405 (2000); https://doi.org/10.1016/S1360-1385(00)01745-3.
  3. A.K. Suresh, R. Sharma, B.K. Bansal, C.J. Singh, Int. J. Res. Develop. Pharmacy Life Sci., 4, 1559 (2015).
  4. P.J. Blankestijn and H. Rupp, Cardiovasc. Hematol. Agents Med. Chem., 6, 253 (2008); https://doi.org/10.2174/187152508785909500.
  5. W.H. Frishman, A. Cheng-Lai and J. Nawarskas, Current Cardiovascular Drugs, Current Medicine Group, p. 53 (2007).
  6. G.S. Devika, M. Sudhakar and J.V. Rao, Pharm. Anal. Acta, 2, 3. (2011).
  7. Indian Pharmacopoeia Vol, II Published by the Government of India, Ministry of Health and Family Welfare, the Indian Pharmacopoeia Commission, New Delhi, pp. 1194-1196 (2007).
  8. British Pharmacopoeia Vol. II, Department of Health and Social Services for Northern Ireland, Stationary Publication, London, p. 330 (2007).
  9. United States Pharmacopoeia National Formulary USP23, Asian Edition, United States Pharmacopoeia Convention, Rockville, MD, USA, pp. 2566-2568 (2009).
  10. T. Anusha, P. Vishnu Priya, N. Anjali Devi, Shyamala, J.V.C. Sharma, Indo Am. J. Pharm. Res., 3, 11 (2013).
  11. D. Sridharan and A. Thenmozhi, Asian J. Pharm. Clin. Res., 3, 93 (2010).
  12. L.R.P. de Abreu, S.A.C. de Castro and J. Pedrazzoli, AAPS PharmSci, 5, 1 (2003); https://doi.org/10.1208/ps050221.
  13. R.K. Barman, M.A. Islam, M. Ahmed, M.I.I. Wahed, R. Islam, A. Khan, M.B. Hossain and B.M. Rahman, Pak. J. Pharm. Sci., 20, 274 (2007).
  14. A.P. Argekar and S.G. Powar, J. Pharm. Biomed. Anal., 21, 1137 (2000); https://doi.org/10.1016/S0731-7085(99)00210-1.
  15. J. Kavitha and S. Muralidharan, Int. J. ChemTech. Res., 2, 880 (2010).
  16. S.R. Sathe and S.B. Bari, Acta Chromatogr., 19, 270 (2007).
  17. T. Sivakumar, P. Venkatesan, R. Manavalan and K. Valliappan, Indian J. Pharm. Sci., 69, 154 (2007); https://doi.org/10.4103/0250-474X.32137.
  18. P. Gupta, C. Issa and A. Bansal, Indian J. Pharm. Sci., 67, 672 (2005);
  19. V. Marinkovic, D. Agbaba, S. Vladimirov and S. Stankovic, J. Pharm. Biomed. Anal., 24, 993 (2001); https://doi.org/10.1016/S0731-7085(00)00531-8.
  20. S.Y. Oh, K.Y. Kim, Y.G. Kim and H. Kim, J. Pharm. Biomed. Anal., 32, 387 (2003); https://doi.org/10.1016/S0731-7085(03)00129-8.
  21. J. Pastera, L. Mejstríková, J. Zoulová, K. Macek and J. Kvìtina, J. Pharm. Biomed. Anal., 44, 674 (2007); https://doi.org/10.1016/j.jpba.2006.06.045.
  22. A.B. Baranda, N. Etxebarria, R.M. Jimenez and R. Alonso, Talanta, 67, 933 (2005); https://doi.org/10.1016/j.talanta.2005.04.028.
  23. A.B. Baranda, R.M. Jimenez and R.M. Alonso, J. Chromatogr. A, 1031, 275 (2004); https://doi.org/10.1016/j.chroma.2003.11.019.
  24. A.P. Argekar and J.G. Sawant, J. Liq. Chromatogr. Relat. Technol., 22, 1571 (1999); https://doi.org/10.1081/JLC-100101752.
  25. M.T. Raju, S. Gurrala, J.V. Rao and K.R. Sambasiva, Int. J. Pharm. Pharmaceut. Sci., 3, (2011).
  26. A.C. Müller and I. Kanfer, J. Pharm. Biomed. Anal., 53, 113 (2010); https://doi.org/10.1016/j.jpba.2010.03.019.
  27. E. Cateau, N. Tournier, A. Dupuis, G. Le Moal and N. Venisse, J. Pharm. Biomed. Anal., 39, 791 (2005); https://doi.org/10.1016/j.jpba.2005.04.025.
  28. A. Loregian, S. Pagni, E. Ballarin, E. Sinigalia, S.G. Parisi and G. Palù, J. Pharm. Biomed. Anal., 42, 500 (2006); https://doi.org/10.1016/j.jpba.2006.04.031.
  29. R.M.W. Hoetelmans, M. van Essenberg, M. Profijt, P.L. Meenhorst, J.W. Mulder and J.H. Beijnen, J. Chromatogr. B: Biomed. Sci. Appl., 705, 119 (1998); https://doi.org/10.1016/S0378-4347(97)00500-8.
  30. K.C. Marsh, E. Eiden and E. McDonald, J. Chromatogr. B: Biomed. Sci. Appl., 704, 307 (1997); https://doi.org/10.1016/S0378-4347(97)00454-4.
  31. N. Sirisha, A. Haripriya, N.S. Bhavani, R. Bhagirath and M. Satyanarayana, Scholars Res. Lib., 5, 78 (2013).
  32. J. Kavitha, J.S.K. Nagarajan, S. Muralidharan and B. Suresh, Int. J. Pharm. Pharmaceut. Sci., 3, 113 (2011).
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