Spectrophotometric Determination of Cefepime

Abstract

Two simple and sensitive spectrophotometric methods (A and B) have been developed for the estimation of Cefepime in pure as well as in pharmaceutical formulations. Method A is based on the formation of blue coloured species with Folin-Ciocalteu reagent under alkaline conditions exhibiting maximum absorbance at 700 nm, Method B is based on the oxidative coupling reaction with 3-methyl-2-benzothiazolinone hydrazone and ferric chloride to form a coloured species with l_max at 630 nm. Beer's law is obeyed at the concentration range of 10–40 μg/mL for method A and 2.5–12.5 μg/mL for method B. These methods have been statistically evaluated and are found to be precise and accurate.