Concurrent Spectrophotometric Assay of Amoxycillin and Ambroxol from Combination Tablets

Abstract

Two simple and economical UV spectrophotometric methods for simultaneous estimation of amoxycillin and ambroxol in multi-component formulations have been developed. Method I is based on Vierdot’s method (simultaneous equations) in which the respective absorbance maximas, i.e., 272 and 308 nm are utilized, whereas, in method II the absorbance differences between 272 and 327 nm and between 308 and 328 nm were utilized for quantification of amoxycillin and ambroxol, respectively. Proper selection of wavelengths eliminates complex interference raised in estimation of one drug by other. The results of analysis have been validated statistically at a 95 per cent confidence level with Student’s-test and by recovery studies. Accurate and reproducible results were obtained in samples containing 100–500 μg/mL of amoxycillin trihydrate and 10–60 μg/mL of ambroxol hydrochloride.