Estimation of Atorvastatin by High Performance Liquid Chromatography in Pure and Pharmaceutical Dosage Forms

Abstract

A reverse phase high performance liquid chromatographic method has been developed for the estimation of atorvastatin in pure form and in pharmaceutical formulations. The quantification was carried out using a RPC-18 column in isocratic mode, with mobile phase consisting of acetonitrile, water and triethyl amine in the ratio of 800 : 200 : 0.2 (v/v). Beclomethasone dipropionate was used as an internal standard. The detection was carried out at 248 nm and the linearity was found to be in the range of 3–15 μg/mL. The proposed method was found to be simple, precise, accurate, less time consuming and reproducible for the estimation of atorvastatin in pharmaceutical dosage forms, i.e., tablets.