Simultaneous Determination of Atorvastatin Calcium and Amlodipine Besylate from Pharmaceutical Formulation by Reversed Phase High Performance Liquid Chromatography

Abstract

A simple, fast and sensitive reverse phase high performance liquid chromatographic method has been developed for the determination of atorvastatin calcium and amlodipine besylate by isocratic elution in its dosage form. These drugs were then chromatographed on a Thermoquest ODS-3 μm column, 250 × 4.6 mm and the detection was carried out at 246 nm. The method was statistically validated for its linearity, accuracy and precision. The intra-day and inter-day variation was found to be less than 0.5% showing high precision of the assay method. The calibration curve was linear in the concentration range 50 to 150% of 200 ppm. Due to its specificity, accuracy/recovery and precision, the proposed method may be used for the determination of atorvastatin calcium and amlodipine besylate in its finished dosage form.