Production and Optimization of Bacterial Cellulose with Different Carbon and Nitrogen Sources Using Gluconacetobacter xylinus C18 Strain

Abstract

Cellulose is eco-friendly homopolysacharide biomacromolecules which are produced by plants and several genera of bacteria. Bacterial cellulose has been biosynthesized mainly by Gram-negative bacteria such as Acetobacter, Pseudomonas, Salmonella, Alcaligenes, Azotobacter, Rhizobium etc., though Gram-positive bacteria Sarcina ventriculi has also been separated for its biosynthesis. Bacterial cellulose having a great potential of it industrial application due to its unique properties such as high purity, high crystallinity, hydrophilicity, elasticity, high water holding capacity, higher degree of polymerization and, good mechanical strength. The present studies focus to evaluate the potential of Gluconacetobacter xylinus C18 at different carbon and nitrogen sources in different condition such as temperature, pH and incubation time. The new strain C18 produced the highest 4.12 g/L of cellulose when we used (2 % w/v) glucose as carbon source and (1 % w/v) yeast extract as nitrogen source were taken. The pH and temperature were maintained as 6.5 and 30 °C, respectively. The bacterial cellulose was characterizationed by FT-IR and scanning electron microscopy.