Purification and Characterization of Peroxidase from Brassica oleracea var. Acephala

Lhami Gulcin; Ali Yildirim

Issue

Vol. 17 No. 4 (2005): Vol 17 Issue 4

Issue Published : August 5, 2005

Purification and Characterization of Peroxidase from Brassica oleracea var. Acephala

Lhami Gulcin

Department of Chemistry, Faculty of Science and Arts, Atatrk University, TR-25240, Erzurum, Turkey

Ali Yildirim

Department of Chemistry Education, Ataturk University, Kazim Karabekir Education Faculty, 25240 Erzurum, Turkey

Corresponding Author(s) : Lhami Gulcin

igulcin@atauni.edu.tr

Asian Journal of Chemistry, Vol. 17 No. 4 (2005): Vol 17 Issue 4
Article Published : June 7, 2016

Abstract

Peroxidase enzyme was extracted and purified from Brassica oleracea var. acephala through ammonium sulfate precipitation, dialysis and CM-Sephadex ion-exchange chromatography. The molecular weight of this enzyme was found to be 95 kDa by an SDS-PAGE electrophoresis. Optimum temperature, optimum pH and stable pH of this enzyme were found as 40°C, pH 7.5 and pH 6.5, respectively. The enzyme had K_M values of 5.5 and 1 mM for guaiacol and H₂O₂, respectively. K_M values for pyrogallol and H₂O₂ was found to be 1.92 and 1.25 mM. In contrast K_M values, each of guaiacol/H₂O₂ and pyrogallol/H₂O₂ pairs were 5000 and 833.33 EU/mL, respectively.

Keywords

Brassica oleracea Peroxidase Purification Enzyme

Gulcin, L., & Yildirim, A. (2016). Purification and Characterization of Peroxidase from Brassica oleracea var. Acephala. Asian Journal of Chemistry, 17(4), 2175–2183. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/13893

Issue

Vol. 17 No. 4 (2005): Vol 17 Issue 4

Purification and Characterization of Peroxidase from Brassica oleracea var. Acephala

Corresponding Author(s) : Lhami Gulcin

Abstract

Keywords

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