Synthesis and Distribution of Bioinspired Silver Nanoparticles Using Spirulina Extract for Control of Vibrio parahaemolyticus Infection in Aquaculture

Abstract

In the present study the antibacterial efficacy of green synthesized silver nanoparticles using Spirulina extract is reported. Synthesis of silver nanoparticles (AgNPs) was achieved by mixing 15 mL of Spirulina extract with 85 mL of 1 mM of silver nitrate solution in a conical flask and incubated at room temperature under stirred condition for 4 days. The synthesized AgNPs were characterized by UV-visible spectroscopy, X-ray diffraction, fourier transform infrared spectroscopy, selected area electron diffraction (SAED), energy dispersive X-ray spectroscopy, field emission scanning electron microscopy and transmission electron microscopy. The biosynthesized silver nanoparticles exhibited a higher antibacterial activity (28 mm) against Vibrio parahaemolyticus at 150 μg/mL. Further, synthesized AgNPs reduces the mortality rate of Artemia nauplii up to 26.6 % by controlling the V. parahaemolyticus infection. This finding confirms the antibacterial potential of Spirulina mediated silver nanoparticles against the aquaculture pathogen V. parahaemolyticus.