Recent Advances and Applications of Turbulent Flow Chromatography

Abstract

Turbulent flow chromatography is a separation technique combined with online extraction of macromolecules in biological samples. Turbulent flow chromatography was basically developed to separate macro molecules from small molecules and analytes without any pretreatment prior to injecting the sample onto the chromatographic system, hence it reduces the time of analysis and also bypasses the manual extraction step of macro molecules in the biological samples. It finds numerous applications as an on-line sample cleanup of biological matrices in preparation of sample and extraction of analytes from biological matrices during estimation of analytes in biological samples and bio-analytical method development. The present work describes the advancements, its wide range of applications as well as advantages and disadvantages of the turbulent flow chromatography over the existing methods.