Spectrophotometric Estimation of Cefetamet Pivoxil in Pharmaceutical Formulations

Abstract

Three new simple, sensitive, selective and economical methods (A, B and C) have been developed for the quantitative estimation of cefetamet pivoxil in bulk and its pharmaceutical formulations. Method A is based on the formation of pink coloured chromoges obtained when drug was dizotised with nitrous acid followed by coupling the resulting diazonium salt with diphenylamine exhibiting λ_max at 521 nm. Method B is based on the redox reaction of drug with Folin-Ciocalteau (FC) reagent exhibiting λ_max at 675.5 nm. Method C is based on the formation of coloured Schiff base obtained when drug reacted with PDAB (p-dimethylaminobenza1dehyde) to form yellow coloured chromogen exhibiting λ_max at 411 nm. These methods obeyed Beer's law in the concentration range of 10-50, 5-25 and 20-100 μg/mL, respectively. The results of analysis for the 3 methods have been validated statistically and by recovery studies. The results are comparable with those obtained with UV spectrophotometeric method in double distilled water at λ_max 234 nm.