Simultaneous Spectrophotometric Estimation of Losartan Potassium and Atenolol in Bulk and Two Component Formulation

Abstract

Two simple, accurate and economical spectrophotometric methods, derivative spectroscopy method and absorption correction method have been developed for the simultaneous estimation of Losartan potassium and atenolol in their combined dosage formulation. Losartan potassium shows absorbance maxima at 205 nm while atenolol shows at 225.5 nm in double distilled water. The first method employs first order derivative spectroscopy to eliminate spectral interferences. The second method is the absorption correction method using 225.5 and 250 nm as the two sampling wavelengths. The methods allow rapid analysis of binary pharmaceutical formulation with accuracy. Both the drugs exhibit linearity with absorbances in the concentration ranges employed for the methods. Results of the methods were validated statistically and by recovery studies.