Spectrophotometric Determination of Lansoprazole and Domperidone in Capsule Formulation

Abstract

Two simple, sensitive and accurate spectrophotometric methods requiring no prior separations have been developed and validated for simultaneous estimation of lansoprazole and domperidone in pharmaceutical formulations. The methods employed are simultaneous equation method and second derivative method. The method A involves formation and solving of simultaneous equation using 287.0 and 294.2 nm as the wavelengths of detection. Method B is second derivative method in which 295.2 nm and 268.6 nm were used as wavelengths for estimation of drugs. Both the methods were validated statistically and recovery studies were carried out. Both the drugs obey Beer's law individually and in mixture within the concentration range of 5-50 μg/mL. Linearity for lansoprazole and domperidone were in the range of 24-36 and 8-12 μg/mL, respectively. The methods were found to be accurate, precise and specific and can be utilized for routine analysis of lansoprazole and domperidone from capsule formulation.