The thin layer chromatographic method described in United State Pharmacopoeia (USP-28) do not quantify in the separation of starting material with thionordiazepam and the substance 2-(2-aceto hydrazinyl)-7-chloro-5-phenyl-3H-1,4 benzodiazapine with alprazolam drug substance. An alternative high performance thin layer chromatographic method is developed for the separation and estimation of the starting material, synthesis related intermediates in alprazolam drug substance. The HPTLC method is capable of detecting the impurities at a level of 0.05 % (with respect to test concentration).
ANJANEYULU, Y., MARAYYA, R., LINGA RAO, D., & KRISHNA RAO, P. (2010). High Performance Thin Layer Chromatographic Determination of the Related Substances in Alprazolam Drug. Asian Journal of Chemistry, 19(5), 3375–3381. Retrieved from https://asianpubs.org/index.php/ajchem/article/view/13213
