HPTLC Method for Simultaneous Estimation of Rabeprazole Sodium and Itopride Hydrochloride in Capsule and Bulk Drug

Abstract

A simple, rapid, sensitive, high performance thin layer chromatography has been developed and validated for simultaneous estimation of rabeprazole sodium and itopride hydrochloride in capsule. It was performed on TLC plate precoated with silica gel 60F₂₅₄ prewashed with methanol as a stationary phase using mobile phase comprising of toluene:chloroform:methanol:ammonia (25 % solution) (25:30:10:1) and the detection was carried out in absorbance at 225 nm. The per cent estimation of labelled claims of rabeprazole sodium and itopride hydrochloride from, market capsule (rabiumplus) formulation was found to be 98.2 and 99.3 %. The method was validated in terms of accuracy, precision, linearity. Linearity was observed between 120-280 μg/mL for rabeprazole sodium and 900-1900 μg/mL for itopride hydrochloride. The recoveries of drugs by standard addition method were found to be in range 98.14 and 99.35 % for both drugs. The proposed method is precise and accurate and can be used for routine analysis of rabeprazole sodium and itopride hydrochloride in capsule formulation.