Validated High Performance Liquid Chromatography Method for Simultaneous Estimation of Paracetamol and Aceclofenac in Tablet Dosage Forms

Abstract

A simple, specific, precise and rapid HPLC method has
been developed for estimation of paracetamol and aceclofenac
simultaneously in tablet dosage form. In this method
standard solution and sample solution of paracetamol and
aceclofenac were applied on C₁₈ reversed phase column with
a mobile phase consisting of methanol, buffer, water and
acetonitrile (85:15:25:5 v/v/v/v) at a flow rate of 1 mL/min
and spectrophotometric detection was carried out at 274 nm.
This HPLC system was quantitatively evaluated in terms of
linearity, accuracy, precision, repeatability and specificity
proving the utility in estimation of drug content in tablet
dosage forms.