An Investigation on the Effects of Nickel(II) p-Hydroxybenzoate on Genomic DNA

Abstract

In this study, an investigation was performed to determine the interaction between genomic DNA and Ni(II) phydroxybenzoate complex. DNA samples have been isolated from the cattle leucocytes and the investigation was carried out by incubating the samples with the Ni complex at 37ºC for varying times. The effect of the Ni(II) complex on genomic DNA was carried out by agarose gel electrophoresis at 50 V for 2 h. The intensity of the bands on the gel changed due to varied concentrations of the complex. The mobility of the bands declined as the concentrations of complex increased, indicating the occurrence of increased covalent binding of the metal complex with DNA. The damage effect of the added ascorbate into the medium might be dependent on the free radicals produced from oxidation of ascorbate by molecular oxygen and this damage is considered to reduced by the metal complex.