HPLC and UV Spectrophotometric Determination of Amrinone Lactate

Abstract

Two assay procedures based on UV spectrophotometry and high performance liquid chromatography (HPLC) have been developed for the determination of amrinone lactate in bulk drug and pharmaceutical formulation (parenteral). UV spectrophotometry involves the determination of amrinone lactate by dissolving it in water followed by measuring absorbance at 259 nm. The HPLC determination was carried out on a reversed phase C-18 column using a mobile phase consisting of water:acetonitril:methanol (40:30:30) at a flow rate of 1.0 mL/min with UV detection at 259 nm. UV Spectrophotometric method is applicable over 2-10 μg/mL range of amrinone lactate with a molar absorptivity of 2.6996 × 10⁴ L mol^-1 cm^-1 and a Sandell's sensitivity of 11.11 ng/cm². In HPLC method a rectilinear relationship was observed between 0.2-40 μg/mL and analysis time was 7 min. The retention time was found to be 2.710 min. The methods when applied to the determination of amrinone lactate in parenteral gave satisfactory results. The developed methods were found to be precise and accurate from the statistical validation of the analysis data.