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High Performance Liquid Chromatographic Method for Simultaneous Quantification of Naloxone and Oxycodone
Corresponding Author(s) : Pallapati Suman
Asian Journal of Chemistry,
Vol. 29 No. 11 (2017): Vol 29 Issue 11
Abstract
High performance liquid chromatography was applied to the simultaneous determination of naloxone and oxycodone. The method depends on separation and analysis of naloxone and oxycodone using C18 analytical column (250 × 4.6 mm, 5 μm). The mobile phase consists of a mixture of potassium dihydrogen phosphate (pH 5.5; 0.1 M) and acetonitrile (70:30, v/v). The photodiode array detector was set at 210 nm. The retention times of naloxone and oxycodone were 2.893 and 4.946 min, respectively. The total run time was 6 min. The developed method was validated as per the guidelines of International Conference on Harmonization and successfully applied to the simultaneous determination of naloxone and oxycodone in their tablets. All the results of validation parameters were acceptable and confirmed that the method is appropriate for the estimation of naloxone and oxycodone simultaneously.
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- Naloxone Hydrochloride, The American Society of Health-System Pharmacists. Retrieved Jan 2, 2015.
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- O. Cheremina, I. Bachmakov, A. Neubert, K. Brune, M.F. Fromm and B. Hinz, Biomed. Chromatogr., 19, 777 (2005); https://doi.org/10.1002/bmc.516.
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References
Naloxone Hydrochloride, The American Society of Health-System Pharmacists. Retrieved Jan 2, 2015.
S. Sirohi, S.V. Dighe, P.A. Madia and B.C. Yoburn, J. Pharmacol. Exp. Ther., 330, 513 (2009); https://doi.org/10.1124/jpet.109.152678.
E.W. Boyer, N. Engl. J. Med., 367, 146 (2012); https://doi.org/10.1056/NEJMra1202561.
J. Riley, E. Eisenberg, G. Müller-Schwefe, A.M. Drewes and L. Arendt- Nielsen, Curr. Med. Res. Opin., 24, 175 (2008); https://doi.org/10.1185/030079908X253708.
R. Al-Hasani and M.R. Bruchas, Anesthesiology, 115, 1363 (2011); https://doi.org/10.1097/ALN.0b013e318238bba6.
T.R. Kosten and T.P. George, Sci. Pract. Perspect., 1, 13 (2002);
http://www.medscape.com/viewarticle/828812.
J. Poelaert, G. Koopmans-Klein, A. Dioh, F. Louis, M. Gorissen, D. Logé, J.V.O. den Bosch and Y.J. van Megen, Clin. Ther., 37, 784 (2015); https://doi.org/10.1016/j.clinthera.2015.02.010.
M.E., Gil-Alegre, M.L. Barone and A.L. Torres-Suárez, J. Sep. Sci., 28, 2086 (2005); https://doi.org/10.1002/jssc.200400068.
R. Panchagnula, P. Sharma, S. Khandavilli and M.V. Varma, IL Farmaco, 59, 839 (2004); https://doi.org/10.1016/j.farmac.2004.06.002.
R.W. Reid, A. Deakin and D.J. Leehey, J. Chromatogr. A, 614, 117 (1993); https://doi.org/10.1016/0378-4347(93)80230-2.
M.S. Tawakkol, M.E. Mohamed and M.M.A. Hassan, J. Liq. Chromatogr., 6, 1491 (1983); https://doi.org/10.1080/01483918308064866.
H. Jiang, Y. Wang, M.S. Shet, Y. Zhang, D. Zenke and D.M. Fast, J. Chromatogr. B, 879, 2663 (2011); https://doi.org/10.1016/j.jchromb.2011.06.039.
W.B. Fang, Y. Chang, E.F. McCance-Katz and D.E. Moody, J. Anal. Toxicol., 33, 409 (2009); https://doi.org/10.1093/jat/33.8.409.
A.W.E. Wright, J.A. Lawrence, M. Iu, T. Cramond and M.T. Smith, J. Chromatogr. B. Biomed. Sci. Appl., 712, 169 (1998); https://doi.org/10.1016/S0378-4347(98)00146-7.
E.M.S. Hannele and K.S. Piia, J. AOAC Int., 83, 1497 (2000).
F. Pantano, S. Brauneis, A. Forneris, R. Pacifici, E. Marinelli, C. Kyriakou, S. Pichini and F.P. Busardò, Clin. Chem. Lab. Med., 55, 1324 (2017); https://doi.org/10.1515/cclm-2016-0990.
O. Cheremina, I. Bachmakov, A. Neubert, K. Brune, M.F. Fromm and B. Hinz, Biomed. Chromatogr., 19, 777 (2005); https://doi.org/10.1002/bmc.516.
M. Neuvonen and P.J. Neuvonen, Ther. Drug Monit., 30, 333 (2008); https://doi.org/10.1097/FTD.0b013e31816e2d4b.
S.G. McKinley, J.J. Snyder, E. Welsh, C.M. Kazarian, M.H. Jamerson and K.L. Klette, J. Anal. Toxicol., 31, 434 (2007); https://doi.org/10.1093/jat/31.8.434.
B.A. Goldberger, C.W. Chronister and M.L. Merves, Methods Mol. Biol., 603, 245 (2010); https://doi.org/10.1007/978-1-60761-459-3_22.
A.B. Wey and W. Thormann, J. Chromatogr. B, 770, 191 (2002); https://doi.org/10.1016/S1570-0232(01)00568-2.
ICH Validation of analytical procedures; Text and methodology; Q2(R1), International Conference on Harmonization (2005)