Simultaneous Spectrophotometric Estimation of Nebivolol Hydrochloride and Hydrochlorothiazide in Tablets

Abstract

Two simple, accurate and precise methods for simultaneous   estimation of nebivolol hydrochloride (NEB-H) and hydrochlorothiazide   (HCT) in combined dosage form have been   described. First method employs formation and solving of   Q-absorbance equation at 275 nm (isoabsorptive point) and   at 282.5 nm (λ_max of NEB-H). The second method involves   formation and solving of simultaneous equation using 282.5   and 271.5 nm (λ_max of HCT) using methanol as solvent. The   result of analysis has been validated statistically and by   recovery studies with standard deviation < 1.0 % was found.   The proposed methods were successfully applied for estimation   of nebivolol hydrochloride and hydrochlorothiazide in combined   tablet formulation.