Issue

Vol. 29 No. 11 (2017): Vol 29 Issue 11

Copyright (c) 2017 AJC

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles Using Leaf Extracts of Carissa carandas

https://doi.org/10.14233/ajchem.2017.20713
Prabhakara Rao Dasari
Department of Chemistry, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad-211 007, India
Prashant Anthony
Department of Chemistry, Sam Higginbottom University of Agriculture, Technology & Sciences, Allahabad-211 007, India

Corresponding Author(s) : Prabhakara Rao Dasari

prabhakararao1986@gmail.com

Asian Journal of Chemistry, Vol. 29 No. 11 (2017): Vol 29 Issue 11

Abstract

In the present study, silver nanoparticles were synthesized from aqueous silver nitrate solution using leaf extracts of Carissa carandas and screening the activity of the synthesized nanoparticles against different microorganisms. Leaf extract was added to aqueous silver nitrate solution. After few hours colour of the solution changed from yellowish to brown. The confirmation of the nanoparticles was done by UV-visible spectroscopy; SEM and TEM analysis were done to further investigate the shape and size of the nanoparticles. TEM images reveal that the average size of the nanoparticles was 28 nm. EDAX was used for the identification of multi-elements. The Carissa carandas plant leaf extracts are capable of producing silver nanoparticles extracellularly. The silver nanoparticles were quite stable in solution. The synthesized nanoparticles were screened for antimicrobial activity on five different microorganisms by agar well diffusion method. The nanoparticles have exhibited good antimicrobial activity against the five bacterial stains which were tested viz. Klebsiella pneumoniae, Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. Out of these, synthesized silver nanoparticles have shown the best inhibition efficiency against Klebsiella pneumoniae.

Keywords

Carissa carandas Silver nanoparticles Green synthesis Antimicrobial activity
Dasari, P. R., & Anthony, P. (2017). Green Synthesis, Characterization and Antimicrobial Activity of Silver Nanoparticles Using Leaf Extracts of Carissa carandas. Asian Journal of Chemistry, 29(11), 2415–2418. https://doi.org/10.14233/ajchem.2017.20713
Download Citation
Endnote/Zotero/Mendeley (RIS)
BibTeX
References
  1. V.K. Sharma, R.A. Yngard and Y. Lin, Adv. Colloid Interface Sci., 145, 83 (2009); https://doi.org/10.1016/j.cis.2008.09.002.
  2. S. Gurunathan, K. Kalishwaralal, R. Vaidyanathan, D. Venkataraman, S.R.K. Pandian, J. Muniyandi, N. Hariharan and S.H. Eom, Colloids Surf. B Biointerfaces, 74, 328 (2009); https://doi.org/10.1016/j.colsurfb.2009.07.048.
  3. V. Parashar, R. Parashar, B. Sharma and A.C. Pandey, Dig. J. Nanomater. Biostruct., 4, 45 (2009).
  4. M. Catauro, M.G. Raucci, F. De Gaetano and A. Marotta, J. Mater. Sci. Mater. Med., 15, 831 (2004); https://doi.org/10.1023/B:JMSM.0000032825.51052.00.
  5. N.A. Begum, S. Mondal, S. Basu, R.A. Laskar and D. Mandal, Colloids Surf. B Biointerfaces, 71, 113 (2009); https://doi.org/10.1016/j.colsurfb.2009.01.012.
  6. M. Starowicz, B. Stypula and J. Banas, Electrochem. Commun., 8, 227 (2005); https://doi.org/10.1016/j.elecom.2005.11.018.
  7. R.M. Tripathi, S. Antariksh, G. Nidhi, K. Hars and R.P. Singh, Dig. J. Nanomater. Biostruct., 5, 320 (2010).
  8. S. Ahmed, M. Ahmad, B.L. Swami and S. Ikram, J. Adv. Res., 7, 17 (2016); https://doi.org/10.1016/j.jare.2015.02.007.
  9. S. Kagithoju, V. Godishala and R.S. Nanna, Biotech, 5, 709 (2015); https://doi.org/10.1007/s13205-014-0272-3.
  10. M. Lengke and G. Southam, Geochim. Cosmochim. Acta, 70, 3646 (2006); https://doi.org/10.1016/j.gca.2006.04.018.
  11. C.J. Murphy, J. Mater. Chem., 18, 2173 (2008); https://doi.org/10.1039/B717456J.
  12. R. Vaidyanathan, K. Kalishwaralal, S. Gopalram and S. Gurunathan, Biotechnol. Adv., 27, 924 (2009); https://doi.org/10.1016/j.biotechadv.2009.08.001.
  13. K. Venugopal, H.A. Rather, K. Rajagopal, M.P. Shanthi, K. Sheriff, M. Illiyas, E. Rather, S. Manikandan, M. Uvarajan, M. Bhaskar and M. Maaza, J. Photochem. Photobiol. B, 167, 282 (2017); https://doi.org/10.1016/j.jphotobiol.2016.12.013.
  14. U.B. Jagtap and V.A. Bapat, Ind. Crops Prod., 46, 132 (2013); https://doi.org/10.1016/j.indcrop.2013.01.019.
  15. N. Ahmad, S. Sharma, V.N. Singh, S.F. Shamsi, A. Fatma and B.R. Mehta, Biotechnol. Res. Int., 2011, 1 (2011); https://doi.org/10.4061/2011/454090.
  16. V. Gopinath, D. MubarakAli, S. Priyadarshini, N.M. Priyadharsshini, N. Thajuddin and P. Velusamy, Colloids Surf. B Biointerfaces, 96, 69 (2012); https://doi.org/10.1016/j.colsurfb.2012.03.023.
  17. P.P.N. Vijay Kumar, S.V.N. Pammi, P. Kollu, K.V.V. Satyanarayana and U. Shameem, Ind. Crops Prod., 52, 562 (2014); https://doi.org/10.1016/j.indcrop.2013.10.050.
  18. T.N.V.K.V. Prasad and E.K. Elumalai, Asian Pac. J. Trop. Biomed., 1, 439 (2011); https://doi.org/10.1016/S2221-1691(11)60096-8.
  19. G.M. Sulaiman, W.H. Mohammed, T.R. Marzoog, A.A.A. Al-Amiery, A.A.H. Kadhum and A.B. Mohamad, Asian Pac. J. Trop. Biomed., 3, 58 (2013); https://doi.org/10.1016/S2221-1691(13)60024-6.
  20. C. Krishnaraj, E.G. Jagan, S. Rajasekar, P. Selvakumar, P.T. Kalaichelvan and N. Mohan, Colloids Surf. B Biointerfaces, 76, 50 (2010); https://doi.org/10.1016/j.colsurfb.2009.10.008.
  21. N. Sahu, D. Soni, B. Chandrashekhar, B.K. Sarangi, D. Satpute and R.A. Pandey, Bioprocess Biosyst. Eng., 36, 999 (2013); https://doi.org/10.1007/s00449-012-0841-y.
  22. P. Malairajan, G. Gopalakrishnan, S. Narasimhan and K.J.K. Veni, Int. J. Drug Dev. Res., 4, 133 (2012).
  23. S. Kaviya, J. Santhanalakshmi, B. Viswanathan, J. Muthumary and K. Srinivasan, Spectrochim. Acta A Mol. Biomol. Spectrosc., 79, 594 (2011); https://doi.org/10.1016/j.saa.2011.03.040.
  24. P. Dibrov, J. Dzioba, K.K. Gosink and C.C. Hase, Antimicrob. Agents Chemother., 46, 2668 (2002); https://doi.org/10.1128/AAC.46.8.2668-2670.2002.
Read More
Author biographies is not available.
Download
PDF
Statistic
Read Counter : 0 Download : 0