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Anticancer Activity of Flavone of Excoecaria agallocha in Cytotoxicity Studies Using Hela Cells
Corresponding Author(s) : Karnati Rajeswari
Asian Journal of Chemistry,
Vol. 29 No. 11 (2017): Vol 29 Issue 11
Abstract
In this study, the compound flavone of mangrove plant Excoecaria agallocha was subjected to cytotoxicity studies using Hela cells was produced from ATCC, stock cells was cultured in Dulbecco's Modified Eagle medium (DMEM) supplemented with 10 % inactivated fetal bovine serum (FBS), penicillin (100 IU/mL), streptomycin (100 μg/mL) in an humidified atmosphere of 5 % CO2 at 37 ºC until confluent. The cell was dissociated with trypsin phosphate versene glucose (TPVG) solution (0.2 % trypsin, 0.02% EDTA, 0.05 % glucose in PBS). The viability of the cells are checked and centrifuged. Further 50,000 cells/well was seeded in a 96 well plate and incubated for 24 h. At 37 ºC, 5 % CO2 incubator, it was observed that while concentration increases, the inhibition increases. Finally, the compound showing good activity in Hela cells. Docetaxel was used as standard drug.
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- K.L. Erickson, J.A. Beutler, J.H. Cardellina, J.B. McMahon, D.J. Newman and M.R. Boyd, J. Nat. Prod., 58, 769 (1995); https://doi.org/10.1021/np50119a020.
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- A.S.R. Anjaneyulu and V. Lakshmana Rao, Phytochemistry, 62, 585 (2003); https://doi.org/10.1016/S0031-9422(02)00269-8.
- A.S.R. Anjaneyulu and V.L. Rao, Phytochemistry, 55, 891 (2000); https://doi.org/10.1016/S0031-9422(00)00251-X.
- T. Konishi, T. Konoshima, T. Maoka and Y. Fujiwara, Tetrahedron Lett., 41, 3419 (2000); https://doi.org/10.1016/S0040-4039(00)00391-9.
- S.P.M. Crouch, R. Kozlowski, K.J. Slater and J. Fletcher, J. Immunol. Methods, 160, 81 (1993); https://doi.org/10.1016/0022-1759(93)90011-U.
- R.J. Gonzalez and J.B. Tarloff, Toxicol. in Vitro, 15, 257 (2001); https://doi.org/10.1016/S0887-2333(01)00014-5.
- N. Hattori, T. Sakakibara, N. Kajiyama, T. Igarashi, M. Maeda and S. Murakami, Anal. Biochem., 319, 287 (2003); https://doi.org/10.1016/S0003-2697(03)00322-1.
- L. Kangas, A.L. Nieminen and M. Grönroos, Med. Biol., 62, 338 (1984).
- A. Lundin, M. Hasenson, J. Persson and Å. Pousette, Methods Enzymol., 133, 27 (1986); https://doi.org/10.1016/0076-6879(86)33053-2.
- Cell Viability and Prolifiration, BioFiles, vol. 6, no. 5, pp. 17-21.
- F. Denizot and R. Lang, J. Immunol. Methods, 89, 271 (1986); https://doi.org/10.1016/0022-1759(86)90368-6.
- T. Mosmann, J. Immunol. Methods, 65, 55 (1983); https://doi.org/10.1016/0022-1759(83)90303-4
References
K.L. Erickson, J.A. Beutler, J.H. Cardellina, J.B. McMahon, D.J. Newman and M.R. Boyd, J. Nat. Prod., 58, 769 (1995); https://doi.org/10.1021/np50119a020.
T. Konishi, M. Azuma, R. Itoga, S. Kiyosawa, Y. Fujiwara and Y. Shimada, Chem. Pharm. Bull. (Tokyo), 44, 229 (1996); https://doi.org/10.1248/cpb.44.229.
T. Konishi, S. Kiyosawa, T. Konoshima and Y. Fujiwara, Chem. Pharm. Bull. (Tokyo), 44, 2100 (1996); https://doi.org/10.1248/cpb.44.2100.
T. Konishi, T. Konoshima, Y. Fujiwara, S. Kiyosawa, K. Miyahara and M. Nishi, Chem. Pharm. Bull. (Tokyo), 47, 456 (1999); https://doi.org/10.1248/cpb.47.456.
Z.C. Wang, Y. Lin, D.Q. Feng, C.H. Ke, P. Lin, C.L. Yan and J.D. Chen, Molecules, 14, 414 (2009); https://doi.org/10.3390/molecules14010414.
A.S.R. Anjaneyulu and V. Lakshmana Rao, Phytochemistry, 62, 585 (2003); https://doi.org/10.1016/S0031-9422(02)00269-8.
A.S.R. Anjaneyulu and V.L. Rao, Phytochemistry, 55, 891 (2000); https://doi.org/10.1016/S0031-9422(00)00251-X.
T. Konishi, T. Konoshima, T. Maoka and Y. Fujiwara, Tetrahedron Lett., 41, 3419 (2000); https://doi.org/10.1016/S0040-4039(00)00391-9.
S.P.M. Crouch, R. Kozlowski, K.J. Slater and J. Fletcher, J. Immunol. Methods, 160, 81 (1993); https://doi.org/10.1016/0022-1759(93)90011-U.
R.J. Gonzalez and J.B. Tarloff, Toxicol. in Vitro, 15, 257 (2001); https://doi.org/10.1016/S0887-2333(01)00014-5.
N. Hattori, T. Sakakibara, N. Kajiyama, T. Igarashi, M. Maeda and S. Murakami, Anal. Biochem., 319, 287 (2003); https://doi.org/10.1016/S0003-2697(03)00322-1.
L. Kangas, A.L. Nieminen and M. Grönroos, Med. Biol., 62, 338 (1984).
A. Lundin, M. Hasenson, J. Persson and Å. Pousette, Methods Enzymol., 133, 27 (1986); https://doi.org/10.1016/0076-6879(86)33053-2.
Cell Viability and Prolifiration, BioFiles, vol. 6, no. 5, pp. 17-21.
F. Denizot and R. Lang, J. Immunol. Methods, 89, 271 (1986); https://doi.org/10.1016/0022-1759(86)90368-6.
T. Mosmann, J. Immunol. Methods, 65, 55 (1983); https://doi.org/10.1016/0022-1759(83)90303-4