Binding of Naphthofuran derivatives to Bovine Serum Albumin by Distribution Experiments

Abstract

The interaction of 2-phenyl-3-p-(β-pyrrolidins)ethoxyphenyl naphthofuran hydrochloride with N,N-diethyl amino derivative to bovine serum albumin (BSA) at pH 7.4 was studied applying partition method. A comparision of binding constants of two types of compounds indicated stronger combination with N,N-diethyl amino derivative than that of pyrrolidine derivative. The intrinsic association constants being 1.62 × 10³ with former and 1.53 × 10³ for the later, respectively. The solvents used for the distribution experiments do not play any significant role deciding the extent of ligand-BSA interactions. The data indeed revealed that the value of n, K and ΔFº was the same for bovine serum albumin either from toluene or from n-octyl alcohol.