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in vitro Antioxidant Activity and Total Phenolic Content of Rhizome Extracts from Acorus calamus Linn.
Corresponding Author(s) : Om Prakash
Asian Journal of Chemistry,
Vol. 29 No. 11 (2017): Vol 29 Issue 11
Abstract
The present study describes antioxidant activities of methanolic extracts from Acorus calamus (Araceae) rhizomes. The antioxidant potential of methanolic extracts were determined by in vitro antioxidant screening models viz. DPPH radical scavenging activity, reductive ability and metal chelating activity along with the estimation of total phenolics, flavonoids and ortho-dihydroxy contents. The total phenolic, flavonoid and ortho-dihydroxy contents estimated ranged from 1.67 ± 0.288 to10.42 ± 0.180 mg/g gallic acid, 0.50 ± 0.053 to 24.50 ± 0.322 mg/g catechin and 0.43 ± 0.145 to 5.50 ± 0.552 mg/g catechol, respectively among different locations. Sample 7th was found best for antioxidant activity (reducing power 119.42 ± 0.630 μg/mL and DPPH 143.35 ± 0.74 μg/mL). The antioxidant activity of the extracts were found to be positively correlated with phenolic content. However IC50 value exhibited negative correlation resulting A. calamus is a possible potential source of natural antioxidant.
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- V.Y.A. Barku, Y. Opoku-Boahen, E. Owusu-Ansah and E.F. Mensah, Asian J. Plant Sci. Res., 3, 69 (2013).
- R. Srinivasan, M.J.N. Chandrasekar, M.J. Nanjan and B. Suresh, J. Ethnopharmacol., 113, 284 (2007); https://doi.org/10.1016/j.jep.2007.06.006.
- S. Manju, R.P. Chandran, P.K. Shaji and G.A. Nair Int. J. Pharm. Pharm. Sci., 5, 4 (2013).
- H.S. Wu, D.F. Zhu, C. Zhou, C.-R. Feng, Y.-J. Lou, B. Yang and Q.-J. He, J. Ethnopharmacol., 123, 288 (2009); https://doi.org/10.1016/j.jep.2009.03.004.
- M.-H. Lee, Y.-Y. Chen, J.-W. Tsai, S.-C. Wang, T. Watanabe and Y.-C. Tsai, Food Chem., 126, 1 (2011); https://doi.org/10.1016/j.foodchem.2010.08.052.
- R. Balakumbahan, K. Rajamani and K. Kumanan, J. Med. Plants Res., 4, 2740 (2010).
- S. Manikandan and R.S. Devi, Pharmacol. Res., 52, 467 (2005); https://doi.org/10.1016/j.phrs.2005.07.007.
- O. Prakash, M. Gondwal and A.K. Pant, Indian J. Nat. Prod. Resour., 2, 4 (2011).
- G.S. Jeena, H. Punetha, O. Prakash, M. Chandra and K.P.S. Kushwaha, Indian J. Nat. Prod., 5, 56 (2014).
- G. Kunwar, O. Prakash, M. Chandra and A.K. Pant, Asian J. Tradit. Med., 8, 88 (2013).
- G. Shui and L.P. Leong, J. Chromatogr. A, 977, 89 (2002); https://doi.org/10.1016/S0021-9673(02)01345-6.
- K. Shetty, O.F. Curtis, R.E. Levin, R. Witkowsky and W. Ang, J. Plant Physiol., 147, 447 (1995); https://doi.org/10.1016/S0176-1617(11)82181-4.
- Y.M. Choi, D.O. Noh, S.Y. Cho, H.J. Suh, K.M. Kim and J.M. Kim, LWT-Food Sci. Technol., 39, 756 (2006); https://doi.org/10.1016/j.lwt.2005.05.015.
- A. Mahadevan and R. Sridhar, In: Methods in Physiological Plant Pathology, Sivikami Publication, Chennai, India, edn 3, pp. 190-192 (1986).
- P. Jayanthi and P. Lalitha, Int. J. Pharm. Pharm. Sci., 3, 126 (2011).
- B.L. Nanda, G.S.N. Sultana and T.T. Radhakrishnan, J. Drug Deliv. Ther., 4, 6 (2014).
- K. Subathraa and T.V. Poonguzhali, Int. J. Curr. Sci., 10, 169 (2012).
- S.A. Devi and D. Ganjewala, J. Herbs Spices Med. Plants, 17, 1 (2011); https://doi.org/10.1080/10496475.2010.509659.
- A. Bahukhandi, S. Rawat, I.D. Bhatt and R.S. Rawal, Natl. Acad. Sci. Lett., 36, 93 (2013); https://doi.org/10.1007/s40009-012-0109-8.
- S. Surveswaran, Y. Cai, H. Corke and M. Sun, Food Chem., 102, 938 (2007); https://doi.org/10.1016/j.foodchem.2006.06.033.
- F. Ahmed, N.S.J.N. Chandra, A. Urooja and K.S. Rangappa, J. Pharm. Res., 2, 830 (2009).
- A. Wojdylo, J. Oszmianski and R. Czemerys, Food Chem., 105, 940 (2007); https://doi.org/10.1016/j.foodchem.2007.04.038.
- V. Kondakova, I. Tsvetkov, R. Batchvarova, I. Badjakov, T. Dzhambazova and S. Slavov, Biotechnol. Biotechnol. Equip., 23, 1444 (2009); https://doi.org/10.2478/V10133-009-0024-4.
- R. Kumar, O. Prakash, A.K. Pant, S.K. Hore, C.S. Chanotiya and C.S. Mathela, Nat. Prod. Commun., 4, 275 (2009).
- N. Joshi, O. Prakash and A.K. Pant, J. Essential Oil Bearing Plants, 15, 32 (2012); https://doi.org/10.1080/0972060X.2012.10644016.
References
V.Y.A. Barku, Y. Opoku-Boahen, E. Owusu-Ansah and E.F. Mensah, Asian J. Plant Sci. Res., 3, 69 (2013).
R. Srinivasan, M.J.N. Chandrasekar, M.J. Nanjan and B. Suresh, J. Ethnopharmacol., 113, 284 (2007); https://doi.org/10.1016/j.jep.2007.06.006.
S. Manju, R.P. Chandran, P.K. Shaji and G.A. Nair Int. J. Pharm. Pharm. Sci., 5, 4 (2013).
H.S. Wu, D.F. Zhu, C. Zhou, C.-R. Feng, Y.-J. Lou, B. Yang and Q.-J. He, J. Ethnopharmacol., 123, 288 (2009); https://doi.org/10.1016/j.jep.2009.03.004.
M.-H. Lee, Y.-Y. Chen, J.-W. Tsai, S.-C. Wang, T. Watanabe and Y.-C. Tsai, Food Chem., 126, 1 (2011); https://doi.org/10.1016/j.foodchem.2010.08.052.
R. Balakumbahan, K. Rajamani and K. Kumanan, J. Med. Plants Res., 4, 2740 (2010).
S. Manikandan and R.S. Devi, Pharmacol. Res., 52, 467 (2005); https://doi.org/10.1016/j.phrs.2005.07.007.
O. Prakash, M. Gondwal and A.K. Pant, Indian J. Nat. Prod. Resour., 2, 4 (2011).
G.S. Jeena, H. Punetha, O. Prakash, M. Chandra and K.P.S. Kushwaha, Indian J. Nat. Prod., 5, 56 (2014).
G. Kunwar, O. Prakash, M. Chandra and A.K. Pant, Asian J. Tradit. Med., 8, 88 (2013).
G. Shui and L.P. Leong, J. Chromatogr. A, 977, 89 (2002); https://doi.org/10.1016/S0021-9673(02)01345-6.
K. Shetty, O.F. Curtis, R.E. Levin, R. Witkowsky and W. Ang, J. Plant Physiol., 147, 447 (1995); https://doi.org/10.1016/S0176-1617(11)82181-4.
Y.M. Choi, D.O. Noh, S.Y. Cho, H.J. Suh, K.M. Kim and J.M. Kim, LWT-Food Sci. Technol., 39, 756 (2006); https://doi.org/10.1016/j.lwt.2005.05.015.
A. Mahadevan and R. Sridhar, In: Methods in Physiological Plant Pathology, Sivikami Publication, Chennai, India, edn 3, pp. 190-192 (1986).
P. Jayanthi and P. Lalitha, Int. J. Pharm. Pharm. Sci., 3, 126 (2011).
B.L. Nanda, G.S.N. Sultana and T.T. Radhakrishnan, J. Drug Deliv. Ther., 4, 6 (2014).
K. Subathraa and T.V. Poonguzhali, Int. J. Curr. Sci., 10, 169 (2012).
S.A. Devi and D. Ganjewala, J. Herbs Spices Med. Plants, 17, 1 (2011); https://doi.org/10.1080/10496475.2010.509659.
A. Bahukhandi, S. Rawat, I.D. Bhatt and R.S. Rawal, Natl. Acad. Sci. Lett., 36, 93 (2013); https://doi.org/10.1007/s40009-012-0109-8.
S. Surveswaran, Y. Cai, H. Corke and M. Sun, Food Chem., 102, 938 (2007); https://doi.org/10.1016/j.foodchem.2006.06.033.
F. Ahmed, N.S.J.N. Chandra, A. Urooja and K.S. Rangappa, J. Pharm. Res., 2, 830 (2009).
A. Wojdylo, J. Oszmianski and R. Czemerys, Food Chem., 105, 940 (2007); https://doi.org/10.1016/j.foodchem.2007.04.038.
V. Kondakova, I. Tsvetkov, R. Batchvarova, I. Badjakov, T. Dzhambazova and S. Slavov, Biotechnol. Biotechnol. Equip., 23, 1444 (2009); https://doi.org/10.2478/V10133-009-0024-4.
R. Kumar, O. Prakash, A.K. Pant, S.K. Hore, C.S. Chanotiya and C.S. Mathela, Nat. Prod. Commun., 4, 275 (2009).
N. Joshi, O. Prakash and A.K. Pant, J. Essential Oil Bearing Plants, 15, 32 (2012); https://doi.org/10.1080/0972060X.2012.10644016.