UV Spectroscopic and Colorimetric Methods for the Estimation of Metadoxine in Tablets

Abstract

Two simple and sensitive spectroscopic methods in ultraviolet and visible region were developed for the estimation of metadoxine in pharmaceutical dosage forms. Method A is based on metadoxine, showing absorbance at 290 nm in distilled water. The method B is based on the reaction of metadoxine with 1.5 % w/v (2 mL) solution of ferric nitrate reagent, to yield a yellow orange colour. This colour has characteristic light absorption in the visible region, with absorption maximum at 445 nm. These methods obeys Beers law in the concentration range of 2 to 28 and 10 to 100 μg/mL, respectively.