Simultaneous Estimation of Chlorhexidine Gluconate, Metronidazole, Lignocaine Hydrochloride and Triamcinolone Acetonide in Combined Dosage Form by RP-HPLC

Abstract

A simple, selective, rapid and precise reverse phase HPLC method has been developed for the simultaneous estimation of chlorhexidine gluconate, metronidazole, lignocaine hydrochloride and triamcinolone acetonide from gel dosage form. The method was carried out on Phenomenex Luna C₁₈ (250 × 4.6 mm, 5 μ) column with a mobile phase consisting of sodium dihydrogen phosphate (pH 3.0 ± 0.05) buffer and acetonitrile programmed for gradient elution at a flow rate of 1 mL/min. The detecting wavelength is 230 nm using Photo diode array (PDA) detector. The retention times of chlorhexidine gluconate, metronidazole benzoate, lignocaine hydrochloride and triamcinolone acetonide were 13.50, 17.98, 10.52 and 16.65, respectively. The proposed method was also validated and it was found to be selective, accurate and precise.