Extraction and Analysis of Volatile Oil of Mussaenda hainanensis Merr. by GC/MS

Abstract

In present study, the chemical constituents of essential oils and relative content of Mussaenda hainanensis Merr. are reported. The essential oils were extracted from Mussaenda hainanensis Merr. by Headspace-solidphase microextraction (HS-SPME) technique and steam distillation (SD). The chemical constituents of essential oils separated were identified by GC/MS analysis, the relative content of the constituents from the essential oils were determined by area normalization method. Chromatographic condition were: Capillary column DB-5 (30 m × 0.25 mm, 0.25 μm); Program temperature: initial column temperature 40 ºC, maintened 2 min and increased to 270 ºC at the rate of 10 ºC min^-1, held 15 min. No split. Injector temperature 280 ºC. There were 37 constituents were separated and identified by Headspace-solid-phase microextraction, which accounted for over 97.45 % of total essential oil fraction. There were 42 constituents were separated and identified by steam distillation, which accounted for over 93.29 % of total essential oil fraction.