RP-HPLC Determination of Pantoprazole Sodium in Tablets

Abstract

A precise reverse phase HPLC method has been developed for the determination of pantoprazole sodium in its tablet dosage forms. The chromatography was carried out on an ODS column using a mixture of acetonitrile and phosphate buffer (32:68 v/v) as the mobile phase at a flow rate of 1.0 mL/min. The detection of the drug in the eluent was done at 280 nm. The retention time of the drug was found to be 11.38 min. The method produced linear responses in the concentration range of 0.5-50 μg/mL of pantoprazole sodium. The method was found to be applicable for determination of the drug in tablets with an average per cent recovery of 99.85.