LC Method for the Determination of Abacavir in Human Plasma for Pharmacokinetic Studies

Abstract

A simple, rapid, sensitive, selective and high performance liquid chromatography method with MS/MS was developed and validated for determination of abacavir in human plasma. Extraction from the plasma was by liquid-liquid extraction. Tenofovir was used as the internal standard. The chromatographic separation was performed by Thermo C18, 4.6 × 50 mm, 5 μm column with mobile phase comprising of (phase pH 5 adjusted with acetic acid) ammonium acetate:acetonitrile (20:80 % v/v). The assay precision ranged from 2.1 to 4.3 and accuracy between 90.3 to 104.8 %, revealing that the method has good reproducibility over the concentration range 20 to 10000 ng mL^-1. The lower limit of quantitation is 20 ng/mL and the recovery of analyte and internal standard (IS) were found to be 62.86-63.62 % and 60.71-62.49 %. Frequently co administered drugs did not interfere with the described methodology. The proposed validated method is suitable to support a wide range of pharmacokinetic/ bioequivalence studies.