Synthesis of Modified Neurotensin on Poly(styrene-co-hexanediol diacrylate) Resin: Quantification of Native Neurotensin in Brain During Diabetics Using Synthetic Peptide

Abstract

Quantification of neurotensin isolated from brain stem, cerebral cortex and hypothalamus of controlled and diabetic rats were carried out. The neurotensin peptide has been synthesized on a 2 % hexanediol dimethacrylate cross-linked polystyrene (PS-HDODA) support. The PSHDODA support was prepared by aqueous free radical suspension polymerization of styrene and 1,6-hexanediol dimethacrylate. The resin was functionalized with aminomethyl group under controlled conditions. The resin with 0.2 mmol/g amino functionality was used for the synthesis of peptide. The acid labile linker, HMPB was incorporated to the resin and the peptide synthesis proceeds following the Fmoc-synthetic strategy. The peptide was purified by HPLC and the identity was exposed by amino acid analysis and mass spectroscopy. The pure peptide was employed for the construction of a standard working curve and the concentration of neurotensin in brain stem, cerebral cortex and hypothalamus of controlled and diabetic rats were determined from the HPLC peak area of the isolated peptide and the working curve. The neurotensin concentration decreases in brain stem and hypothalamus where as it increases in cerebral cortex during diabetics.