Spectroscopic and HPTLC Method for Quantification of Simvastatin in Tablet Dosage Forms

Abstract

Two simple methods, ultra violet spectroscopy and high performance thin layer chromatography for the determination of simvastatin in tablet dosage form are described. Detection wavelength for spectrophotometric and high performance thin layer chromatography methods was found to be 239 nm. For the spectrophotometric method, the linearity was found to be in the range of 3-15 μg/mL with correlation co-efficient of 0.9995 and for the high performance thin layer chromatography; The linear regression analysis data for the calibration plots showed good linear relationship with correlation co-efficient r² = 0.9974 in the concentration range 200-500 ng per spot The average recovery was found to be 99.61 ± 0.29 % and 99.89 ± 0.31 % for spectrophotometric and high performance thin layer chromatography, respectively. The drug was satisfactorily resolved with R_f value 0.602 ± 0.03 in HPTLC method.