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Vol. 29 No. 9 (2017): Vol 29 Issue 9

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Development and Validation of High Performance Liquid Chromatography Method for Simultaneous Estimation of Curcuminoids from Rhizome Extracts

https://doi.org/10.14233/ajchem.2017.20749%20
Amrin Begum
CSIR-Indian Institute of Chemical Technology, Hyderabad-500007, India
Bolla Nagamani
Department of Pharmaceutics, Viswanadha Institute of Pharmaceutical Sciences, Visakhapatnam-531 173, India
Garapati Sridevi
Department of Chemistry, Andhra University College of Engineering for Women, Visakhapatnam-530 017, India

Corresponding Author(s) : Garapati Sridevi

sridevi_g_22@yahoo.co.in; sridevigarapati5@gmail.com

Asian Journal of Chemistry, Vol. 29 No. 9 (2017): Vol 29 Issue 9

Abstract

A rapid and sensitive liquid chromatographic method is developed and validated for the simultaneous estimation of various curcuminoids. The method was used for the estimation of curcuminoids in commercially available dosage forms, bulk and naturally available root and rhizome extracts. The separation was performed on a Phenomenex ODS 2 C18 (5 μm, 15 cm × 4.6 mm) column. The composition of mobile phase is 50:50 % (v/v) of acetonitrile and water containing 2 % v/v glacial acetic acid operated on isocratic mode at a flow rate of 1.2 mL/min. Quantitation was achieved by HPLC-UV detection at 425 nm. The specificity of the method (blank interference, interference from degradation products and interference from formulation excipients) was established. Degradation studies were performed in acid, alkaline, oxidative and photolytic stress conditions. The results showed that curcumin is prone to degradation in all aqueous media irrespective of pH of the solution. Also, in comparison to the standard curcuminoid composition (83.5 % curcumin, 14.5 % dimethoxycurcumin and 1.5 % bis-dimethoxycurcumin), large variation was observed in the root and rhizome powder extracts that were collected from commercial and crop samples.

Keywords

Curcumin bis-Demethoxycurcumin Demethoxycurcumin Rhizome extracts HPLC
Begum, A., Nagamani, B., & Sridevi, G. (2017). Development and Validation of High Performance Liquid Chromatography Method for Simultaneous Estimation of Curcuminoids from Rhizome Extracts. Asian Journal of Chemistry, 29(9), 2031–2037. https://doi.org/10.14233/ajchem.2017.20749
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