Determination of Folic Acid in Syrian Cereals by High Performance Liquid Chromatography with UV-DAD Detection After SPE Extraction

Abstract

Folic acid is a water soluble vitamin which has been determined in some Syrian cereals such as (flour, rice, semolina, corn, gram, borghol, lentil, triticum durm, triticum aestivum, lima bean) by HPLC-DAD detection after solid phase extraction clean up. For analytical conditions, we studied the folic acid determination by C₈ and ODS columns. Isocratic system was used for C8 column. The mobile phase was with a volumetric mixture of 92 % A and 8 % B. The second one for ODS column, the separation was in a gradient system. The flow rate for both columns were 1 mL/min, the wavelength was 280 nm. The concentrations of folic acid varied from 0.02-1.90 μg/g.