Spectrophotometric Determination of Glutathione Based Inhibitory Effect on Hemoglobin

Abstract

A high sensitive and simple catalytic spectrophotometric method for the determination of reduced glutathione based on its inhibitory effect on the hemoglobin-cayalyzed the reaction of H₂O₂ and acid chrome blue K(ACBK) was developed. The concentration of glutathione is linear with the percentage inhibition (I %) of system under the optimal experimental conditions. The calibration graph is linear in range 1.82 × 10^-7 to 4.55 × 10^-5 mol L^-1 with the detection limit of 3.49 × 10^-8 mol L^-1. The relative standard deviation was 3.97 % for 11 determinations of 1.82 × 10^-5 mol L^-1. This method can be used for the determination of glutathione in pharmaceuticals and vegetables with satisfactory results.